甲基紫精胁迫下转GLP7基因大豆抗氧化性的初步分析  

Analysis of Antioxidant System Activities in Transgenic Soybean Transformed with GLP7 under Methyl Viologen Stress

作　　者：李维娜[1] 李永光[1] 盖江南[1] 王朋朋[1] 王涛[1] 李文滨[1] 

机构地区：[1]大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室/东北农业大学大豆研究所,黑龙江哈尔滨150030

出　　处：《大豆科学》2013年第2期149-153,共5页Soybean Science

基　　金：抗逆转基因大豆新品种培育重大专项(2011ZX08004-002)

摘　　要：为探讨GLP7基因参与植物抗氧化胁迫的作用机制及其生物学功能,以转GLP7基因大豆T2代株系及其受体品种垦丰16为材料,在氧化剂甲基紫精(MV)胁迫条件下,测定植株叶绿素、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的变化情况。结果表明:相对于非转基因大豆,转基因大豆在MV氧化胁迫下MDA含量降低,总叶绿素含量增加,SOD、CAT活性显著提高。初步证明GLP7的过量表达提高了大豆的抗氧化能力,降低了氧化胁迫对大豆幼苗的损伤。To discuss the function mechanism of GLP-I gene in anti-oxidation stress and its biological function, GLP7 transgenic lines of soybean and its acceptor cultivar Kenfeng 16 （WT） were used to measure activities of superoxide dismutase（ SOD）, cat- alase（CAT） ,chlorophyll and（MDA） contents under oxidant methyl viologen （MV）stress. The results showed that, compared with non-transgenic soybeans, transgenie soybeans reduced MDA content,increased total chlorophyll content, SOD and CAT ac- tivity in MV stress treatment. These results indicated that the over expression of GLP7 could effectively enhance the activities of antioxidant ability and reduce the damage on the soybean seedlings under oxidative stress.

关 键 词：GLP7基因 大豆 MV 氧化胁迫 叶绿素 抗氧化酶 

分 类 号：S565.1[农业科学—作物学]