大豆GmGAMYB1启动子克隆与分析  

Isolation and Analysis of Promoter of GmGAMYB1 from Soybean

作　　者：谷月娇[1] 赵琳[1] 张彦威[1] 卢清瑶[1] 宋仙萍[1] 李文滨[1] 

机构地区：[1]大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室/东北农业大学大豆研究所,黑龙江哈尔滨150030

出　　处：《大豆科学》2013年第2期160-164,共5页Soybean Science

基　　金：国家自然科学基金(31101169;30671318;31271748);黑龙江省教育厅大豆分子设计团队项目;黑龙江省教育厅省高校青年学术骨干项目;东北农业大学博士基金

摘　　要：利用PCR技术从大豆品种东农42基因组中分离得到了GmGAMYB1基因启动子片段pGmGAMYB1,定向替换载体pBI121的CAMV35S组成型启动子,构建表达载体pBI121-pGmGAMYB1,驱动下游报告基因GUS基因表达,并利用PLACE和PlantCARE在线启动子预测工具进行分析。结果表明:序列中含有多种典型的光及各种激素和胁迫诱导特异性表达元件,如光应答元件如3-AF1 binding site、BOX-4、T-Box和TCT-motif;赤霉素应答元件GARE;脱落酸应答元件ABRE;热激元件CCAATBox;节律钟Ciradian等。Soybean（ Glycine max）is a kind of typical plant in the developmental biology and molecular biology research, the completion of soybean genome has provided a lot of convenience for research, but there is still so little achievement on the soy- bean highly effective inducible promoters. Gibberellin （GA） biosynthesis and signaling pathways have been discussed in detail in recent years. In addition ,several reviews discuss general mechanisms of morphogenesis. GAMYB1 gene is an important pro- motive factor in the signal transduction pathways of GA. In this study ,the promoter of GmGAMYB1 was cloned from the soybean Dongnong 42 by PCR. It was directionally replaced CAMV 35S constitutive promoter of the vector pBI121. The expression vec- tor of pBI121-pGmGAMYB1 was constructed and the expression of downstream reporter GUS gene was drived. The analysis on promoter sequence by online promoter forecasting tool PLACE and PlantCARE showed that sequence contained a variety of specific expression elements, such as light and various hormones and stress-induced elements. GUS histochemical staining was used to explore the role of the promoter in plant growth regulation. This study had laid a good foundation for GrnGAMYB1 char- acteristics temporal and spatial expression.

关 键 词：GmGAMYB1启动子 GUS基因 组织化学染色 

分 类 号：S565.1[农业科学—作物学]