响应曲面法优化南板蓝根中总生物碱的提取工艺  

Optimization of Extraction Technology for Total Alkaloids from Baphicacanthus cusia by Response Surface Methodology

作　　者：刘家水[1] 李世杰[1] 张丹雁[1] 陈奕龙[1] 郑少燕[1] 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2013年第9期41-44,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2010B030700067)

摘　　要：目的:优化南板蓝根中总生物碱的提取工艺。方法:在单因素试验基础上,选取提取时间、乙醇体积分数和液料比3个因素进行Box-Benhnken中心组合设计,以总生物碱提取率为因变量,利用响应曲面法对其提取工艺参数进行优化。结果:优选的提取工艺为提取时间20.23 min,乙醇体积分数83.21%,液料比21.16∶1,南板蓝根总生物碱的最高理论提取率达0.781%。根据生产实际情况,将其修正为提取时间20.5 min,乙醇体积分数83.0%,液料比21∶1,总生物碱提取率(0.78±0.024)%,与理论值较为接近。结论:优化的提取工艺准确可靠,具有一定的实用价值。Objective：To optimize extraction technology of total alkaloids from Baphicacanthus cusia.Method：Based on single factor test,effects of extraction time,ethanol concentration and liquid-solid ratio on yield of total alkaloids from B.cusia was investigated with a three-factor Box-Behnken center-united design,extraction parameters were optimized by response surface methodology.Result：Optimized extraction technology conditions was as following：extraction time 20.23 min,the concentration of ethanol 83.21 %,liquid-solid ratio 21.16∶ 1,theoretical yield of total alkaloids could be up to 0.781%.According to actual situation in production,optimum extraction parameters were modified as extraction time of 20.5 min,the concentration of ethanol 83.0%,liquid-solid ratio 21.0∶ 1,validated experiment showed yield of total alkaloids was（0.78 ± 0.024）% under these conditions,which was in consistency with the theoretic value.Conclusion：This modified extraction parameters were credible and reliable with some practical value.

关 键 词：南板蓝根 总生物碱 响应曲面法 提取工艺 

分 类 号：R283.6[医药卫生—中药学]