不同生产期苦玄参叶HPLC指纹图谱  

HPLC Fingerprint of Leaf of Picria fel-tarrae in Different Growth Periods

作　　者：丘琴[1] 苏春妹[1] 甄汉深[1] 

机构地区：[1]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2013年第9期104-107,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科技厅攻关项目(桂科攻0235022-4);国家中医药管理局民族项目(04-05ZM01);广西教育厅课题[桂教科研([2003]22号]

摘　　要：目的:建立广西产不同生长期苦玄参叶的HPLC指纹图谱。方法:采用高效液相色谱法(HPLC),C18柱(4.6 mm×250 mm,5μm,Japan Shimadzu)分离分析,以乙腈-0.1%冰醋酸溶液为流动相,梯度洗脱,检测波长264 nm,柱温25℃,流速1 mL.min-1,采用"中药色谱指纹图谱相似度评价系统"(2004A)软件进行分析。结果:10批苦玄参叶的HPLC指纹图谱共标定17个共有峰,相似度均>0.9。结论:建立苦玄参叶质量控制方法,指导苦玄参药材的采收加工和规范化种植提供实验数据。Objective：To study the HPLC fingerprint of leaf of Picria fel-tarrae in different growth periods.Method：High performance of liquid chromatography（HPLC） was adopted,the separation was performed on C18（4.6 mm×250 mm,5 μm） column with acetonitrile-0.1% aqueous acetic acid solution as mobile phase using a gradient elution;the detection wavelength was set at 264 nm,the column temperature was set at 25 ℃ and the flow rate was 1 mL · min-1;the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM（Version 2004A）.Result： In the fingerprint,17 common peaks were confirmed and the similarity of 10 batches of leaf of Picria fel-tarrae.were above 0.9.Conclusion： The experiment result could provide experimental data for the quality control method of the leaf of Picria fel-tarrae.and for optimum collection time and actualizing standard planting of Picria fel-tarrae.

关 键 词：苦玄参叶 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]