清肺抑火片指纹图谱的建立及其在质量控制中的应用  

Fingerprinting of Qingfei Yihuo Tablets and its Application in Quality Control

作　　者：王琳[1] 郭冬琴[2] 周浓[1,2] 谢静 徐静[1] 

机构地区：[1]大理学院药学院,云南大理671000 [2]重庆三峡学院生命科学与工程学院,重庆万州404000 [3]重庆市食品药品检验所,重庆江北401121

出　　处：《中国实验方剂学杂志》2013年第9期114-117,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立清肺抑火片HPLC指纹图谱。方法:采用UltrasphereTM-ODS-C18柱(4.6 mm×250 mm,5μm),流动相0.1%磷酸溶液(A)-乙腈(B)梯度洗脱,检测波长270 nm,流速1.0 mL.min-1,柱温40℃。结果:不同厂家的清肺抑火片指纹图谱变异范围差异显著,同一厂家的产品质量基本稳定在一定水平内。结论:该方法可用于测定不同来源清肺抑火片的色谱指纹图谱,并为其全面质量评价和生产工艺监控提供了可行的方法。Objective：To establish the HPLC fingerprint of Qingfei Yihuo Tablets.Method： Ultrasphere TM-ODS-C18 column（4.6 mm ×250 mm,5 μm） was used,the mobile phase was 0.1% phosphoric acid solution（A）-acetonitrile（B） gradient elution,the detection wavelength was set at 270 nm.The flow rate was 1 mL ·min-1,and column temperature was kept at 40 ℃.Result： Fingprints of the tablets from different manufacturers varies significantly,which keep stable at a certain level from the same manufacture.Conclusion： The method can be used for the determination of different source of Qingfei Yihuo Tablets and can provide a feasible method for comprehensive quality evaluation and production process monitoring.

关 键 词：清肺抑火片 指纹图谱 高效液相色谱 相似度 

分 类 号：R284.1[医药卫生—中药学]