玉郎伞多糖对快速老化小鼠额叶、海马神经元丢失和Tau蛋白磷酸化的影响  

Effects of Millettia pulcha Polysaccharides on Neuronal Loss and Tau Phosphorylation in Frontal Lobe and Hippocampus of SAMP8 Mice

作　　者：陈晓宇[1] 荣延平[1] 郭又嘉[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021

出　　处：《中国实验方剂学杂志》2013年第9期181-184,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30960504)

摘　　要：目的:研究玉郎伞多糖(YLSP)对快速老化模型小鼠(SAMP8)额叶、海马神经元丢失和Tau(Ser202)异常磷酸化的影响。方法:选择6月龄SAMP8,随机分为模型组,石杉碱甲组(0.02 mg.kg-1),YLSP低、高剂量组(45,180 mg.kg-1),选择6月龄正常老化小鼠(SAMR1)作为对照组,以上各组每日上午灌胃给药l次,连续40 d,于末次给药的次日采用尼氏染色方法检测额叶和海马神经元缺失率;应用免疫组织化学方法检测额叶和海马Tau(Ser202)磷酸化水平。结果:模型组额叶、海马神经元缺失率分别为(46.4±8.4)%,(45.5±7.2)%。与模型组相比,石杉碱甲组(35.8±6.7)%,(34.2±5.6)%、YLSP低、高剂量组(35.1±6.4)%,(36.0±4.5)%,(25.6±6.6)%,(26.7±6.1)%额叶、海马神经元缺失率明显降低(P<0.05或P<0.01);模型组额叶、海马pSer202阳性细胞数(92.2±10.7),(98.6±11.4)个较对照组明显升高(12.3±2.1),(15.5±2.6)个(P<0.01),与模型组相比,石杉碱甲组(48.5±6.4),(56.1±3.3)个、YLSP低、高剂量组(60.7±7.5),(65.9±8.0);(50.9±5.1),(53.6±6.7)个的额叶、海马Tau(pSer202)阳性细胞数明显降低(P<0.01)。结论:YLSP降低Tau蛋白异常磷酸化,减少脑部神经元缺失。Objective：To study the effects of Millettia pulcha polysaccharides（YLSP） on neuronal loss and Tau（Ser202） phosphorylation in the frontal lobe and hippocampus of SAMP8 mouse.Method： The SAMP8 mice were adopted as AD model group,and the SAMR1 mice as the control group.Both groups were randomized and given the blank configuration solution.Huperzine（0.02 mg · kg-1） was given to drug-positive group and two YLSP groups were given 45,180 mg · kg-1 respectively,continuous administration for 40 days.In the frontal lobe and hippocampus,neuronal loss and Tau（Ser202） phosphorylation（pser202） were respectively detected by Nissl staining and immunohistochemical method.Result： Comparing with the normal group,the neuronal loss and the number of pSer202 positive cells of the model group were higer（P＆lt;0.01） in the frontal lobe and hippocampus.In drug-positive and YLSP groups,the neuronal loss and the number of pSer202 positive cells in the frontal lobe and hippocampus were observed obviously lower（P＆lt;0.05 or P＆lt;0.01）,comparing with the model group.Conclusion： The abnormal Ser202 phosphorylation is reduced and neuronal loss of brain is ameliorated by YLSP.

关 键 词：玉郎伞多糖 快速老化模型小鼠 TAU蛋白磷酸化 神经元缺失 

分 类 号：R285.5[医药卫生—中药学]