不同饲料配方对大鼠TC,LDL-C,Hcy的影响  

Effect of Different Feeds on TC,LDL-C,Hcy in Rats

作　　者：李然[1] 刘建勋[2] 李磊[2] 史跃[2] 马彦雷[2] 

机构地区：[1]河南中医学院,郑州450008 [2]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中国实验方剂学杂志》2013年第9期207-210,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重大新药创制专项(2012ZX09301002-004);国家自然科学基金项目(81073085)

摘　　要：目的:探讨合适的饲料配方,在较短的实验周期内形成稳定、适用的高脂高同型半胱氨酸血症(hyperhomocysteinemia,HHcy)动物模型。方法:40只雄性SD大鼠分为4组,每组10只,分别按组给予普通饲料、配方1(基础饲料占88.5%,高脂高胆固醇不添加丙硫氧嘧啶)、配方2(较配方1增加5%蛋黄糖,5%全脂奶粉,0.15%丙硫氧嘧啶)、配方3(0.25%丙硫氧嘧啶,较配方2增加5%蛋黄粉)饲料常规喂养。每周称1次体重,观察体重变化。分别于2,4周取血测定血清中总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)变化及血浆同型半胱氨酸(homocysteine,Hcy)的浓度。结果:与对照组相比配方1组体重没有明显差异;配方2组,3组体重显著下降(P<0.01)。2,4周时与对照组相比,血清TC和LDL-C含量及血浆Hcy浓度,配方1组没有显著性差异;配方2组,3组都有显著升高(P<0.01)。结论:该实验研究调整后的大鼠饲料配方2组是比较合适的饲料配方组成,能在较短的时间内形成稳定的大鼠高脂HHcy血症动物模型。Objective：We explore a practical feed formulation in order to establish a stable atherosclerosis animal model.With high fat and hyperhomocysteinemia in shorter period.Method：Forty male SD rats were divided into 4 groups（n=10）,given different formula feeding with common feed,formulationⅠ（not containing propylthiouracil）,formulation II（containing propylthiouracil of 0.15%） and formulation Ⅲ（containing propylthiouracil of 0.25%）.weighed once a week.The blood samples were collected at week 2 and 4 respectively,then to determine the levels of total cholesterol（TC）,low density lipoprotein cholesterin（LDL-C） in serum and homocysteine（Hcy） in plasma.Result： Compared with the control group,weight of formulationⅠhad no significant difference,while weight of formulation II and formulation Ⅲ decreased significantly（P＆lt;0.01）;TC,LDL-C and Hcy levels in formulation II and formulation Ⅲ groups increased significantly（P＆lt;0.01） at 2,4 weeks,respectively,but formulationⅠhave no significant difference.Conclusion： In this study,we found that the rats fed with the formulation II can establish a stable atherosclerosis animal model with high fat and hyperhomocysteinemia in shorter period.

关 键 词：动脉粥样硬化 血脂 同型半胱氨酸 

分 类 号：R285.5[医药卫生—中药学]