远志水提取物对家兔心肌收缩力及心房尿钠肽分泌的影响  

Aqueous Extract of Polygala tenuifolia Willd on Atrial Dynamics and ANP Secretion in Beating Rabbit Atria

作　　者：崔昊震[1] 刘丽萍[2] 

机构地区：[1]延边大学中医学院临床基础教研室,吉林延吉133000 [2]延边大学基础医学院生理学教研室,吉林延吉133000

出　　处：《中国实验方剂学杂志》2013年第9期256-259,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(3026003);延边大学科技发展计划项目(601010055)

摘　　要：目的:观察远志水提取物对家兔心肌收缩力及心房尿钠肽分泌的影响。方法:采用家兔离体搏动心房灌流模型,观察远志水提取物对家兔心肌收缩力及心房尿钠肽分泌的影响。环磷酸腺苷(cAMP)和心房尿钠肽(ANP)含量的测定均采用放射免疫法。结果:远志水提取物可使家兔的心房每回搏出量、搏动压和灌流液中cAMP流出量比给药前呈现剂量依赖性递增趋势(P<0.05)。同时,它还可以明显地抑制ANP的分泌(P<0.01)。在阻断β-肾上腺素受体(β-adrenorecptor,β-AR)及选择性阻断β1-AR时能显著抑制远志水提取物对心肌收缩力的增强作用(P<0.05);而选择性阻断β2-AR时却无任何影响。此外,阻断L-型Ca2+通道及选择性抑制蛋白激酶A(PKA)的活性能明显抑制远志水提物对心肌收缩力的增强作用(P<0.05)。结论:远志水提取物主要通过影响β1-AR-cAMP-Ca2+信号传导途径增强心肌收缩力,并对ANP分泌具有抑制作用。Objective：To investigate the effect of aqueous extract of Polygala tenuifolia（APT） on myocardial contractility and atrial natriuretic peptide（ANP） secretion in beating rabbit atria.Method： The APT-induced changes in atrial dynamics,cAMP efflux and ANP secretion were determined in perfused beating rabbit atria.The changes of cAMP and ANP were measured by radioimmunoassay.Result： APT increased atrial stroke volume,pulse pressure and cAMP efflux in a concentration-dependent manner with inhibition of ANP secretion in beating rabbit atria.The APT-induced positive inotropic effect was significantly blocked by pretreatment with blockade of β and β1-adrenoceptors（AR）（P＆lt;0.05） but not β2-AR.Furthermore,the APT-induced changes in myocardial contractility were attenuated by inhibition of L-type Ca2＋channel and protein kinase A（PKA） activation with no changes in cAMP efflux.Conclusion： APT could increase atrial dynamics via β1-AR-cAMP-Ca2＋signaling and decreases ANP secretion in beating rabbit atria.

关 键 词：远志水提取物 心肌收缩力 尿钠肽 β1-肾上腺素受体-cAMP-Ca2+信号传导途径 

分 类 号：R285.5[医药卫生—中药学]