Burkholderia Cepacia脂肪酶在氨基功能化离子液体修饰的SBA-15上的共价固定  

Covalent Immobilization of Burkholderia Cepacia Lipase on Amine Functionalized Ionic Liquid Modified SBA-15

作　　者：胡燚[1,2] 杨姣[1] 唐苏苏[1] 初旭明[1] 邹彬[1] 黄和[1,2] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009 [2]材料化学工程国家重点实验室,南京210009

出　　处：《高等学校化学学报》2013年第5期1195-1202,共8页Chemical Journal of Chinese Universities

基　　金：国家杰出青年科学基金(批准号:21225626);国家自然科学基金(20906049,20936002);国家“八六三”计划项目(批准号:2011AA02A209)资助

摘　　要：合成了氨基以及氨基功能化离子液体修饰的介孔材料SBA-15(NH2-SBA和NH2-IL-SBA),并以戊二醛为活化剂对NH2-IL-SBA进行活化处理(CA-NH2-IL-SBA),通过元素分析、N2吸附-脱附、X射线衍射、红外光谱等方法研究了修饰及活化对SBA-15结构的影响.将所得新型固定化载体用于Burkholderia cepacia脂肪酶(BCL)的吸附固定、共价交联固定及聚集包被固定.以三乙酸甘油酯的水解为模型反应,考察了固定化BCL的酶活、最适反应条件、稳定性等酶学性质.结果表明,离子液体修饰后的载体保持了原有的孔道结构,与氨基修饰以及原粉SBA-15吸附固定的BCL(BCL-NH2-SBA和BCL-SBA-15)相比,其固定化酶的比活力和稳定性都得到了明显提高,对温度及低pH的敏感性降低.其中聚集包被固定的BCL在获得了相对较高酶负载量的同时显示了最好的稳定性,其热稳定性和重复使用性分别为BCL-SBA-15的4倍和2倍.The （3-aminopropyl）trimethoxysilane and amine functionalized ionic liquid modified mesoporous silica SBA-15 （NH2-SBA, NH2-IL-SBA） were synthesized, and NH2-IL-SBA was activated by glutaraldehyde （ CA-NH2-IL-SBA）. Physical and chemical properties of the materials were characterized by elemental analy- sis, nitrogen adsorption-desorption, small-angle X-ray diffraction and Fourier transform infrared spectroscopy. The prepared materials were used as novel carrier systems to immobilize Burkholderia cepacia lipase（BCL） by physical adsorption, conventional covalent attachment and enzyme-aggregate coating methods. Enzymatic properties, including activity, optimum reaction condition and stability were investigated in the triacetin hy- drolysis reaction. The results showed that the modification and activation did not destroy the structure of SBA- 15. Compared with the immobilized BCL on NH2-SBA and parent SBA-15 （BCL-NH2-SBA, BCL-SBA-15） , the immobilized BCL on novel carriers were more resistant to temperature and low pH changes and possessed both higher activity and stability. Especially, the BCL prepared by enzyme-aggregate coating method exhibited highest immobilization efficiency and stability, which improved 4.0 and 2.0 folds of thermal stability and reusability than BCL-SBA-15, respectively.

关 键 词：BURKHOLDERIA cepacia脂肪酶 离子液体 介孔材料 修饰 固定化 

分 类 号：O647[理学—物理化学]