长期接触低剂量氯化甲基汞致脑发育损伤大鼠动物实验模型的研究  

Research on brain damage of rat models caused by long-term exposure to low doses of methylmercury chloride

作　　者：郭杰[1] 张雯艳[2] 刘忠山[1] 李志超[3] 王铁君[1] 邢程[4] 

机构地区：[1]吉林大学第二医院放疗科,吉林长春130041 [2]吉林省妇幼保健院 [3]吉林大学公共卫生学院 [4]吉林省卫生厅

出　　处：《中国妇幼保健》2013年第13期2132-2134,共3页Maternal and Child Health Care of China

基　　金：吉林省卫生厅资助课题〔3D5097923427〕;吉林大学基本科研业务费专项基金资助课题〔421030140427〕;吉林大学青年教师基金资助课题〔419070210048〕

摘　　要：目的:观察大鼠长期接触低剂量氯化甲基汞(MMC)不同发育阶段仔鼠脑汞含量。方法:雌性Wistar大鼠体重(120±20)g,随机分为3个实验组和对照组各30只。实验组母鼠从妊娠前90天至仔鼠出生后30天连续喂饲含有不同剂量(0.75、1.50和3.00 mg/kg)MMC的普通饲料,对照组给予普通饲料。采用干烧法用F732-V冷原子吸收测汞仪分别测定仔鼠大脑、小脑和海马组织汞含量。结果:在建立MMC所致脑发育损伤动物实验模型过程中,发现实验组母鼠的产子率低于正常鼠,高剂量组尤为明显,生后仔鼠外观无异常表现,不同脑区(大脑、小脑和海马)脑汞含量差异无统计学意义(P>0.05)。各实验组生后不同时间仔鼠脑汞含量明显高于相应对照组(P<0.05),各实验组仔鼠脑汞含量随母鼠接触剂量的增高而有不同程度升高(P<0.05),同一实验组内仔鼠脑汞含量随生后时间的延长逐渐升高,在生后30天达峰值。结论:甲基汞可透过血脑屏障蓄积于仔鼠脑内,且仔鼠脑汞含量与母鼠接触剂量之间存在一定剂量反应关系。Objective： To observe the rat offspringt brain mercury content at different developmental stages after long - term expo- sure to low doses of methylmercury chloride （MMC）. Methods： 120 female Wistar rats （120 ±20） g were randomly assigned to the exper- imental groups, fed with common fodder containing various amounts of MMC （0. 75, 1.50 and 3.00 mg/kg） for 90 days before gestation to 30 d post parturition and one control group without MMC. Dry method was used with F732 - V Automated Mercury Analyzer to measure the pups＇mercury content in cerebrum, cerebellum and hippocampus. Results： The number and weight of pups of Exp I were nearly the same with that of control group, however which were lower in Exp I1 and Exp m, pups of different experimental group were normal in the appear- ance, there were no differences in MMC concentration among three brain regions （cerebra, cerebellum and hippocampus） within groups （ P 〉 0. 05 ）. The concentrations of Hg2 ＋ in pups＇brain of experimental groups at various postnatal days were markedly higher than those of the control group （ P 〈 O. 05 ） . Time - course studies in experimental group showed the concentrations of Hg2 ＋ in pups＇brain increased gradually during development and reached its peak at PND30. Conclusion： MMC could pass blood -brain barrier and accumulate in pups＇brain. The enhanced effect is dose- dependent.

关 键 词：氯化甲基汞 毒性 大鼠 

分 类 号：R722[医药卫生—儿科]