紫花苜蓿组织培养体系的建立及其遗传转化  

Establishment of Alfalfa Tissue Culture System and Research on Agrobacterium-mediated Transformation System in Alfalfa

作　　者：周晓馥[1] 吕杰[1] 苗璐[1] 高峰[1] 徐洪伟[1] 

机构地区：[1]吉林师范大学植物资源科学与绿色生产吉林省重点实验室,四平136000

出　　处：《生物技术通报》2013年第4期63-68,共6页Biotechnology Bulletin

基　　金：国家科技部农业科技成果转化资金项目(2010GB2B100118);吉林省科技发展计划项目(20100803);吉林省高技术产业发展项目[吉发改高技(2009)633号];吉林师范大学研究生创新科研计划资助项目[研创新(学)(201116号)]

摘　　要：为建立高效的农杆菌介导的紫花苜蓿的遗传转化体系,以紫花苜蓿无菌苗叶片作为外植体,用8种含有不同激素浓度梯度的培养基对其进行愈伤组织及不定芽的诱导,结果表明,M7培养基(MS+1 mg/L 2,4-D+2 mg/L CH+0.25 mg/L KT+2mg/L琼脂)的愈伤组织诱导率最高,可达100%;继代表现最好,为胚性愈伤组织;同时,继续培养后可直接分化出不定芽,分化率达100%。结果表明,最适宜条件分别为卡那霉素(Kan)筛选浓度为75 mg/L;农杆菌菌液OD_(600)值为0.45-0.6之间。In order to establish high-efficiency alfalfa genetic transformation system, the influence factors of Agrobacterium-mediated transformation system were studied. The callus and adventitious bud of alfalfa were induced by culturing leaves as explants in 8 different kind of medium, named M1-M8, respectively. The results showed that M7 medium （ MS＋I mg/L 2,4-D ＋ 2 mg/L CH ＋ 0.25 mg/L KT ＋ 2 mg/L agar ） was the best one which can induced the much more ammount of callus and adventitious bud of alfalfa than others. The results showed that the most suitable conditions for kanamycin was 75 mg/L; OD600 of agrobactium concerntration value for 0.45-0.6.

关 键 词：紫花苜蓿 组织培养 高频再生遗传转化农杆菌 卡那霉素OD600值 

分 类 号：S541.9[农业科学—作物学]