甘蓝型油菜下胚轴和带柄子叶再生体系研究  

Research on the Regeneration System from Hypocotyls and Cotyledon of Brassica napus L.

作　　者：郝晓云[1] 沈海涛[1,2] 李鸿彬[1,2] 

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]石河子大学 农业生物技术重点实验室,石河子832003

出　　处：《生物技术通报》2013年第4期69-74,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30860031,31260339);农业部转基因重大专项(2009ZX08005-027B)

摘　　要：以甘蓝型油菜野油19号的下胚轴和带柄子叶为外植体,研究其下胚轴和带柄子叶在不同浓度激素配比下的分化率及再生频率的变化。该品系的油菜下胚轴和带柄子叶在1.5 mg/L的2,4-D中预培养4 d后,转入分化培养基中,其愈伤组织形成早,发生快,再生频率和分化频率均较高。其中,下胚轴转入MS+3 mg/L 6-BA+0.1 mg/L NAA培养基中的分化率和再生频率最高,再生频率达到86.67%;带柄子叶外植体的再生频率要比下胚轴的再生频率低,在MS+4 mg/L 6-BA+0.3 mg/L NAA的分化培养基中芽的再生频率为46.67%。本研究初步建立了甘蓝型油菜野油19号的高频率再生体系。To study the changes in the hypocotyls and quizzing cotyledon differentiated and plant regeneration frequency under different concentrations of hormone combinations, hypocotyls and cotyledons with petiole of Brassica napus L. （ wild-oil 19 ） were taken as explants. The results showed that, after pre-cultured for 4 days using 1.5 mg/L 2, 4-D and transferred to differentiated medium of hypocotyls and cotyledons with Petiole, the derived callus initiated earlier and developed faster with a higher induced frequency. Among them, the hypocotyls transferred to the MS ＋3 mg/L 6-BA ＋0.1 mg/L NAA as differentiated medium reached the highest regeneration frequency of 86.67% ; However, the regeneration frequency of cotyledon explants was much lower than hypocotyls with a final regernation frequency of 46.67% using MS with 4 mg/L 6-BA and 0.3 mg/L NAA as differentiated medium. In this study, the high frequency regeneration system of Brassica napus L. （ wild-oil 19 ） was established.

关 键 词：甘蓝型油菜 下胚轴 带柄子叶 再生频率 分化频率 

分 类 号：S565.4[农业科学—作物学]