溶杆菌属Lysobacter yanansis sp. nov.胞内外蛋白双向电泳条件优化及图谱建立  

Optimization and Construction of the Intracellular and Extracellular Proteomic Map of Lysobacter yanansis sp.nov.

作　　者：王斐斐[1] 武坤毅 郭玲[1] 崔浪军[1] 任靖[1] 

机构地区：[1]陕西师范大学生命科学学院陕西师范大学药用植物资源与天然药物化学教育部重点实验室,西安710062

出　　处：《生物技术通报》2013年第4期140-146,共7页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31101481);中央高校基本科研业务费专项资金(GK200902032);2012年度陕西师范大学实验技术研究立项项目(SYJS201213);陕西师范大学青年项目(201001005);陕西师范大学2011年校级大学生创新实验计划项目(cx11072)

摘　　要：通过对溶杆菌属细菌Lysobacteryanansis sp.nov.胞外蛋白上样量和聚焦程序两个方面进行双向凝胶电泳条件的优化,分析了其对溶杆菌Lysobacteryanansis sp.nov.胞外分泌蛋白的双向凝胶电泳图谱的影响。结果表明,150μg的上样量和聚焦程序Ⅱ获得的双向电泳图谱蛋白点数较多且清晰。同时,建立了溶杆菌属细菌Lysobacteryanansis sp.nov.胞外和胞内蛋白双向电泳图谱,经过硝酸银染色和PDQuest图像分析检测到了100种胞外蛋白和204种胞内蛋白。In this study, we separated the extracellular protein excreted by Lysobacter yanansis sp. nov. through the two-dimensional （ 2-D ） electrophoresis, and analyzed the effect of isoelectric focusing programs and loading volumes on 2-D proteomic maps. The results showed that a clearly 2-DE map was obtained when the 150 μg loading volumes and isoelectric focusing programsⅡ were used. Furthermore, 2-D proteomic maps of extract intracellular and extracellular protein from Lysobacter yanansis sp. nov. were constructed. The separated protein spots in gels were analyzed by PDQuest. 100 spots of extracellular proteins and about 204 spots of intracellular proteins had been separated by 2-DE.

关 键 词：溶杆菌属 双向电泳 生物防治 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]