日本蟾蜍MCL-1 cDNA的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of MCL-1 cDNA of Bufo japonicus formosus

作　　者：张姝芳[1] 袁进强[1] 黄慧[1] 诸葛慧[1] 徐跃[1] 杨仙玉[1] 

机构地区：[1]浙江农林大学林业与生物技术学院,临安311300

出　　处：《生物技术通报》2013年第4期179-184,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31071181);2011年度浙江省科技创新活动计划项目(2011R412042)

摘　　要：克隆日本蟾蜍(Bufo japonicus formosus)髓细胞白血病基因-1(myeloid cell leukemia-1,mcl-1),并进行序列分析。通过菌落PCR对日本蟾蜍皮肤cDNA质粒文库筛选获得目的基因。运用生物信息学方法对该基因进行分析,预测其编码蛋白的结构和功能。序列分析表明mcl-1基因(GenBank登录号为JX219482)的cDNA全长为1 090 bp,5′端和3′端非翻译区域分别为19bp和432 bp,ORF由639个碱基组成,编码由212个氨基酸残基组成的蛋白质。经氨基酸序列同源性比对发现,日本蟾蜍与非洲爪蟾(Xenopus laevis)的同源性为60%,与其他7种动物的同源性在38%-55%之间。MCL-1氨基酸序列构建的进化树与传统动物分类学相符。日本蟾蜍MCL-1与人类同源蛋白具有相似的结构特征,对其cDNA序列的进一步分析,将为研究其生物学功能和药物研发奠定基础。It was to clone mcl-1 （ myeloid cell leukemia-1 ） gene from Bufojaponicusformosus and analyze the sequence of the gene. The plasmid cDNA library of adult Japanese toad Bufo japonicus formosus skin was screened by bacterial colony PCR. The sequence was analyzed by bioinformatics method, and the structure and function of MCL-1 were predicted. Result showed that the full length cDNA of mcl-1 （ GenBank accession number ： JX219482 ） was 1 090 bp. The 5′- and 3′-untranslated regions are 19 bp and 432 bp, respectively, while the ORF is 639 bp encoding 212 amino acid residues. Homologous analysis indicated the similarity of Bufo japonicus formosus with Xenopus laevis was 60%, and 38%-55% with other species. The phylogenetic tree of MCL-1 amino acid sequences was consistent with morphological taxonomic characters. The analysis indicates that the structural characteristics of MCL-1 of B. japonicusformosus seem to be similar with those of human. This report would become the basis for the further studies on MCL-1.

关 键 词：日本蟾蜍 髓细胞白血病基因-1 基因克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]