背根神经节小胶质细胞活化在大鼠术后持续性痛形成中的作用  

Role of microgliai activation in dorsal root ganglia in a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction

作　　者：应彦璐[1] 许学兵[2] 余守章[1] 许立新[1] 阮祥才[1] 

机构地区：[1]广州医学院附属广州市第一人民医院麻醉科,510180 [2]广东省第二人民医院麻醉科

出　　处：《中华麻醉学杂志》2013年第2期156-158,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81271247）;广州市卫生局重点课题（201102A212021）

摘　　要：目的评价背根神经节小胶质细胞活化在大鼠术后持续性痛形成中的作用。方法成年雄性sD大鼠70只，体重200—250g，采用皮肤肌肉切口牵拉法建立术后持续性痛模型，采用随机数字表法，将其分为2组（n=35）：假手术组（S组）和术后持续性痛组（SMIR组）。分别于术前1d、术后1、3、7、12、22和32d时，测定机械痛阈。上述各时点痛阈测试完毕后，随机取5只大鼠，计数背根神经节小胶质细胞。结果与s组比较，SMIR组术后3—22d时机械痛阈降低，术后3～12d时背根神经节小胶质细胞计数升高（P〈0．05）。结论背根神经节小胶质细胞的活化可能参与了大鼠术后持续性痛的形成。Objective To evaluate the role of microglial activation in dorsal root ganglia in a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction （SMIR） .Methods Seventy male Spra- gue-Dawley rats, weighing 200-250 g, were randomly divided into 2 groups （n = 35 each） ： group sham operation （group S） and group SMIR. The rat model of persistent postoperative pain evoked by SMIR was established accord- ing to the method described by Flatters. Pain behavior was assessed by mechanical paw withdrawal threshold to von Frey filament stimulation at 1 day before and 1, 3, 7, 12, 22 and 32 days after operation. Five animals were sac rificed at each time point in each group for microglia count in dorsal root ganglia. Results Compared with group S, mechanical paw withdrawal threshold was significantly decreased at 3-22 days after operation, and microglia count was significantly increased at 3-12 days after operation in group SMIR （P 〈 0.05）. Conclusion Microglial activation in dorsal root ganglia may be involved in the development of SMIR-evoked persistent postoperative pain in rats.

关 键 词：小神经胶质细胞 疼痛 手术后 神经节 脊 

分 类 号：R6[医药卫生—外科学]