大鼠妊娠前后吸入异氟烷或七氟烷对子代海马NMDA受体表达的影响  

Effect of isoflurane or sevoflurane inhalation before and after gestation on the N-methyI-D-aspartate recep- tor expression in offspring rat hippocampus

作　　者：王宇恒[1] 成永霞[2] 艾春雨[3] 孙晓峰[1] 焦晶华[1] 赵宏[1] 王俊科[3] 

机构地区：[1]沈阳医学院奉天医院麻醉科,沈阳市110024 [2]牡丹江医学院病理学教研室 [3]中国医科大学附属第一医院麻醉科

出　　处：《中华麻醉学杂志》2013年第2期197-200,共4页Chinese Journal of Anesthesiology

基　　金：2013年沈阳市卫生局课题（2013067）

摘　　要：目的探讨大鼠妊娠前后吸人异氟烷或七氟烷对子代海马N-甲基-D-天冬氨酸（NMDA）受体表达的影响。方法雌性SD大鼠30只，3月龄，体重250-300g，采用随机数字表法，将其分为5组（n=6）：对照组（c组）、妊娠前1d吸入1．6％异氟烷6h组（BI组）、妊娠6、10、14和18d时吸入1．6％异氟烷6h组（PI组）、妊娠前吸入2．4％七氟烷6h组（BS组）和妊娠6、10、14和18d吸入2．4％七氟烷6h组（PS组）。于出生当天（T1）、出生后第7（T2）、14（T3）和28天（T4）时各组按窝别随机取12只子鼠处死取海马，采用免疫荧光和RT-PCR法检测NMDA受体（NR1、NR2A和NR2B）的表达水平。结果与Cc组比较，BI组和BS组子鼠海马NMDA受体表达差异无统计学意义（P〉0．05），PI组和PS组L1-3时子鼠海马NR1和NR2A表达上调，NR2B表达下调，NR1 mRNA和NR2AmRNA表达上调，NR2BmRNA表达下调（P〈0．01），n时NMDA受体表达差异无统计学意义（P〉0．05）；与PI组比较，PS组T1-3时子鼠海马NR1和NR2A表达上调，NR2B表达下调，NR1 mRNA和NR2AmRNA表达上调，NR2BmRNA表达下调（P〈0．05或0．01），Td时NMDA受体表达差异无统计学意义（P〉0．05）。结论妊娠前吸入异氟烷或七氟烷对子鼠海马NMDA受体表达无影响，妊娠后吸入异氟烷或七氟烷可引起子鼠海马NMDA受体表达异常，可能引起子鼠海马神经细胞凋亡和神经系统发育及认知功能异常。Objective To evaluate the effect of isotlurane or sevoflurane inhalation before and after gesta- tion on the N-methyl-D-aspartate （NMDA） receptor expression in offspring rat hippocampus.Metbods Thirty fe- male adult Sprague-Dawley rats, aged 3 months, weighing 250-300 g, were randomly assigned into 5 groups （ n= 6 each）： control group （group C）, exposure to isoflurane before gestation group （group BI）, exposure to isoflu- rane during gestation period group （group PI）, exposure to sevoflurane before gestation group （group BS）, expo- sure to sevoflurane during gestation period group （group PS）. The rats inhaled 1.6% isoflurane for 6 h at I day before gestation in group BI. The rats inhaled 1.6% isoflurane for 6 h at 6, 10, 14 and 18 day gestation in group PI. The rats were exposed to 2.4% sevoflurane for 6 h before gestation in group BS. The rats Were exposed to 2.4% sevoflurane for 6 h at 6, 10, 14 and 18 day gestation in group PS. Twelve offspring rats from pregnant rats in each group were chosen on the day of birth （T1）, and 7th, 14th and 28th days after birth （T2-4） and sacrificed, and the hippoeampi were then isolated for determination of the expression NMDA receptor （NR1, NR2A and NR2B） .Results Compared with group C, no significant change was found in NMDA receptor expression in off-spring rat hippocampus in groups BI and BS （P 〉 0.05）, and the expression of NR1 and NR2A protein and mRNA was significantly up-regulated, and the expression of NR2B protein and mRNA was down-regulated at T1-3 （P 〈 0. 05 ）, and no significant change was found in NMDA receptor expression at T4 in groups PI and PS （ P 〉 0.05）. Compared with group PI, the expression of NRI and NR2A protein and mRNA was significandy up-regulated, and the expression of NR2B protein and mRNA was down-regulated at T1-3 （ P 〈 0.05 or 0.01 ）, and no significant change was found in NMDA receptor expression at T4 in group PS （ P 〉 0.05）. Conclusion Isoflurane or sevoflu- rane inhalation

关 键 词：异氟醚 麻醉药 吸入 妊娠 动物 受体 N-甲基-D-天冬氨酸 海马 

分 类 号：R7[医药卫生—临床医学]