理气化痰祛瘀法对非酒精性脂肪性肝炎大鼠肝组织SREBP-1c/FAS表达的影响  

Effects of Method of Regulating Qi to Dissipate Blood Stasis and Phlegm with Chinese Herbs on Expression of SREBP-1c/FAS in Rats Liver Tissue with Non-Alcoholic Steatohepatitis

作　　者：石占利[1,2] 陈芝芸[1] 严茂祥[1] 何蓓晖[1] 洪研[1] 

机构地区：[1]浙江中医药大学第一临床医学院,浙江杭州300053 [2]浙江省中西医结合医院,浙江杭州310003

出　　处：《中华中医药学刊》2013年第5期1062-1065,I0004,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(Y2100170);浙江省教育厅资助项目(20020080)

摘　　要：目的:观察理气化痰祛瘀中药对高脂饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠肝组织SREBP-1c/FAS表达的影响,探讨其防治NASH的作用机理。方法:以高脂饮食喂养SD大鼠12周建立NASH模型,以不同剂量的理气化痰祛瘀中药复方进行干预,常规HE染色观察肝组织的病理改变并计算NAFLD活动度积分(NAS),RT-PCR法检测肝组织SREBP-1c、FASmRNA表达,免疫组化EnVison法检测SREBP-1c蛋白水平,E-LIAS法测定FAS蛋白水平。结果:模型组大鼠NAS较正常组明显增高,不同剂量的理气化痰祛瘀方组大鼠肝组织的NAS较模型组明显减低(P<0.05);模型组大鼠肝组织SREBP-1c、FASmRNA表达及相应蛋白含量较正常组明显增高(P<0.01),应用不同剂量理气化痰祛瘀方干预后,大鼠肝组织SREBP-1、FAS cmRNA表达及相应蛋白含量较模型组明显降低(P<0.05);肝组织NAS与SREBP-1c及FASmRNA表达均呈明显正相关(r=0.826,0.703;P<0.01);肝组织SREBP-1cmRNA与FASmRNA表达亦呈明显正相关(r=0.613,P<0.01)。结论:SREBP-1c/FAS mRNA表达是非酒精性脂肪性肝炎发生的重要病机,也是理气化痰祛瘀法的重要作用靶向,据此能够改善肝脏脂质代谢紊乱,防止NASH进展。Objective：To investigate the method of regulatinh qi to dissipate blood stasis and phlegm with Chinese herbs on the expression of SREBP-1c/FAS in rats liver tissue with non-alcoholic steatohepatitis.Method：The model of non-alcoholic steatohepatitis was induced by high fat diet to SD rats for twelve weeks,and treated by different doses of Chinese medicine compound with functions of regulatinh qi to dissipate blood stasis and phlegm.The pathological changes of liver tissue were observed with HE staining to calculate NAFLD activity score,and the SREBP-1c/FASmRNA expression of liver tissue were measured by RT-PCR.The SREBP-1c protein quantity was measured by Immunohistochemistry EnVison and the FAS protein by ELIAS.Result： The NAFLD activity scores of rats in model group were significantly higher than that of normal group,and the NAFLD activity scores in Chinese medicine compound groups were all improved（P0.05）.Compared with normal group,the SREBP-1c,FASmRNA expression and protein quantity expression of liver tissues in model group were increased significantly（P0.01）,and decreased markedly after the Chinese medicine compound intervention（P0.05）.The NAS of liver tissue was positively correlated with the expression of SREBP-1c and FASmRNA（r=0.826,0.703;P0.01）,and the expression of SREBP-1cmRNA was also significantly correlated with that of FASmRNA（r=30.613,P0.01）.Conclusion： The expression of SREBP-1c/FASmRNA has an important effect on non-alcoholic steatohepatitis pathogenesis,which is also an important target affected by the method of regulating qi to dissipate blood stasis and phlegm with Chinese herbs.The method can improve the lipid metabolism disorders and prevent the deterioration of NASH.

关 键 词：非酒精性脂肪性肝炎 免疫组化 固醇调节元件结合蛋白 脂肪酸合成酶 

分 类 号：R285.5[医药卫生—中药学]