肿瘤临床中中医药治疗的机理研究  

Study of Functions of Traditional Medicine in Clinical Tumor Treatment

作　　者：郑雪勇 冯联忠 李斌 赵广法[2] 

机构地区：[1]嘉兴第二医院肿瘤外科,浙江嘉兴314000 [2]复旦大学附属肿瘤医院腹部外科,上海200000

出　　处：《中华中医药学刊》2013年第5期1171-1172,共2页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:讨论中药抗肿瘤疗效和Fas/FasL基因对医疗的作用。方法:将肝癌细胞用0、0.01、0.1、1μg/mL表阿霉素一一处理,把上述处理的肝癌细胞和有无参处理的淋巴细胞放在一起培养,观察放入中药或fasl抗体之后对肿瘤的效果;用PCR对化疗药物处理的肝癌细胞Fas/Fasl的具体表达测定,结果:未处理的肝癌细胞OD值最高,而用1μg/mL表阿霉素处理的肝癌细胞OD值高于0,0.01,0.1μg/mL处理的。对于0μg/mL表阿霉素干预的7402肝癌细胞fasl表达对比,7402肝癌细胞表达fas和fasl基因,0.01,0.1μg/mL的降低、1μg/mL的增高;fasl单克隆抗体介入之后,0,0.01,0.1μg/mL表阿霉素处理的肝癌细胞的OD值也高于空白对照。结论:中药抗肿瘤可以增效减毒;Fas/FasL系统在抗肿瘤免疫机制中扮演着重要的角色。Objective：To study the effects of traditional Chinese medicine in the tumor treatments and the functions of Fas/FasL genes.Methods：The lung cancer cells were disposed by 0,0.01,0.1,1 μg/mL pharmoribicin and lymphocyte which had or not had astragalus and then the actual antibody functions of tumor treatment with FasL monoclonal or traditional Chinese medicine were observed;by means of PCR,the actual explanation of lung cancer cell Fas/FasL genes was tested.As a result,the OD of lung cancer cell with 1 μg/mL pharmoribicin was higher than that with 0,0.01,0.1 μg/mL pharmoribicin and the highest was in that without pharmoribicin.Compared with those disposed in 0 μg/mL pharmoribicin and Fas and FasL genes in 7402 lung cancer cells,those in 1 μg/mL was increased while those in 0.01,0.1 μg/mL decreased;after the introduction of FasL monoclonal antibody,the OD in lung cancer cells disposed by 0,0.01,0.1 μg/mL pharmoribicin was also higher than the original.Conclusion： Traditional Chinese medicine can reduce the toxicity in tumor treatment and Fas/FasL plays an important part in anti-tumour immune mechanism.

关 键 词：肿瘤细胞 中医药 DMEM Fas、FasL 淋巴细胞 

分 类 号：R285.5[医药卫生—中药学]