半胱氨酸封闭剂为电化学探针的非标记型核酸适配体传感器  

Label-free Aptamer Biosensor Based on Cysteine Blocker as the Electrochemical Redox Probe

作　　者：杨绍明[1] 查文玲[1] 李红[1] 孙清[1] 刘斌[1] 郑龙珍[1] 

机构地区：[1]华东交通大学基础学院化学化工系,南昌330013

出　　处：《应用化学》2013年第5期549-554,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(21065004);江西省自然科学基金(2009GQH0022);江西省教育厅科技项目(GJJ12304)资助

摘　　要：采用半胱氨酸为封闭剂和氧化还原电化学探针,制备了一种新型的非标记型电化学核酸适配体传感器,并用于测定凝血酶。利用电化学阻抗对传感器的组装过程进行了监测。用循环伏安法和差分脉冲伏安法研究了该传感器的电化学行为。探讨了凝血酶孵育时间、测试pH值对传感器响应的影响。该传感器对凝血酶在10.0～10000μg/L范围内呈良好的线性响应,检测限为2.47μg/L。A novel label-free electrochemical aptamer biosensor was developed by using cysteine blocker as the electrochemical redox probe, and was used for the determination of thrombin. Electrochemical impedance spectroscopy was used to monitor the self-assembly process of aptamer biosensor. The electrochemical behaviors of the biosensor were characterized by the cychc voltammetry and differential pulse vohammetry. The effects of the incubation time and test pH were investigated on the response of aptamer biosensor. The biosensor shows a good linear range of 10. 0 - 10000 μg/L for thrombin with a detection limit of 2. 47 μg/L.

关 键 词：半胱氨酸 封闭剂 氧化还原探针 核酸适配体 生物传感器 

分 类 号：O657.1[理学—分析化学]