逍遥散对慢性束缚应激下脑区NMDA受体NR2A和NR2B表达的影响  

Influence of Xiaoyao powder on expression of NR2A and NR2B in brain NMDA receptor by chronic restraint stress

作　　者：王竹风[1] 汪宝军[1] 岳广欣[2] 陈家旭[3] 张巧丽[3] 许筱颖[3] 

机构地区：[1]中国中医科学院广安门医院南区内分泌科,北京102618 [2]中国中医科学院基础研究所,北京100700 [3]北京中医药大学基础医学院,北京100029

出　　处：《北京中医药》2013年第4期300-304,共5页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672578);高等学校博士学科点专项科研基金(2006110801)

摘　　要：目的观察海马及杏仁核(BLA)N-甲基-D-天冬氨酸(NMDA)受体NR2A和NR2B蛋白在束缚应激状态下的表达变化及逍遥散对其的调节作用。方法使用捆绑的方法制作慢性束缚应激动物模型,并用逍遥散进行干预,分别于7日后和21日后检测模型大鼠海马CA1区、CA3区、齿状回(DG)及BLA中NMDA受体NR2A和NR2B的蛋白表达情况。结果 NR2A和NR2B在海马的CA1、CA3、DG区和BLA中均有不同程度表达。逍遥散21日组海马CA1、CA3、DG区和BLA中NR2A和NR2B表达均上调(P<0.05或P<0.01);海马CA3和DG区中尤其明显(P<0.01)。结论逍遥散对慢性束缚应激引起的海马和BLA神经传递改变有明显的调节作用,可影响突触可塑性,适应机体的功能,主要靶点在海马的CA3和DG区。Objective To observe the change of protein expressions of NR2A and NR2B in N-methyl-D-aspartic （NMDA） receptor in hippocampus and basolateral lateral amygdaloid （BLA） on restraint stress as well as the regulation action of Xiaoyao powder. Methods With tie-up method to establish animal model of chronic restraint stress, Xiaoyao powder was applied to intervene. The expression status of proteins in CA1, CA3, dentate gyrus （DG） of hippocampus, and NR2A, NR2B in NMDA receptor of BLA were tested after 7 and 21 days respectively. Results There was expression of NR2A and NR2B to varying degrees in CA1, CA3, DG of hippocampus and BLA. The expression of NR2A and NR2B in the Xiaoyao powder group on Day 21 was all increased in CA1, CA3, DG and BLA （P 〈 0.05, P 〈 0.01）, which was especially obvidus in CA3 and DG of hippocampus （P 〈 0.01）. Conclusion The Xiaoyao powder has obvious regulation action on chronic restraint stress-induced neurotransmission change in hippocampus and BLA, which could make an influence on plasticity of synapsis to satisfy body function, and the main target is CA3 and DG in hippocampus.

关 键 词：逍遥散 束缚应激 突触可塑性 NR2A蛋白 NR2B蛋白 

分 类 号：R285.5[医药卫生—中药学]