加热回流提取牡丹花粉黄酮工艺研究及其安全性评价  

Reflux extraction and safety evaluation of flavonoids from peony pollen

作　　者：杨丽[1] 王昌涛[1] 孙啸涛[1] 苏丹 

机构地区：[1]北京工商大学植物资源重点实验室,北京100048 [2]中国检验检疫科学研究所中德联合化妆品研究所有限公司,北京100123

出　　处：《日用化学品科学》2013年第4期35-40,共6页Detergent & Cosmetics

基　　金：北京市科技新星项目(2008B08);"中青年骨干人才培养计划"项目(PHR20110873);质检公益性行业科研专项(项目编号201010023)

摘　　要：利用单因素和正交试验,对加热回流提取牡丹花粉总黄酮工艺进行优化。最佳工艺是:100%的乙醇为溶剂,料液比为1:20,水浴温度为90℃,水浴时间为1h,牡丹花粉总黄酮得率为8.28%。化妆品刺激性/腐蚀性鸡胚绒毛尿囊膜眼刺激试验结果表明,其不具有眼刺激性。人体斑贴试验结果表明30位志愿者在反应0.5h、24h和48h内,测试区均呈现阴性反应。The extraction process of peony pollen flavonoids was investigated,and the optimum conditions of the reflux method were studied through orthogonal experiment.The results showed that the optimal condition was as following： 1：20 of material：solvent ratio（W/V）,100% of ethanol solution as solvent,1h of extraction time at 90℃ of bath temperature.The yield of peony pollen flavonoids was 8.28%.The data of cosmetics ocular irritant and corrosive HET-CAM tests were evaluated as no eye irritation.Human skin patch test results showed that all 30 volunteers gave negative reaction in test area after 0.5h,24h and 48h of application.

关 键 词：化妆品 牡丹花粉 工艺 回流 安全性 

分 类 号：TQ914[化学工程]