乌鲁木齐市2011年婴幼儿腹泻轮状病毒感染监测  

Surveillance for rotavirus diarrhea among children in Urumqi in 2011

作　　者：杨学磊[1] 刘冬梅[2] 贾娜尔[1] 余亮[1] 依不拉音[1] 王慧琴[1] 吴卫东[1] 

机构地区：[1]新疆维吾尔自治区人民医院,新疆乌鲁木齐830001 [2]石河子大学医学院

出　　处：《中国预防医学杂志》2013年第4期265-268,共4页Chinese Preventive Medicine

基　　金：国家自然科学基金(30760232);益普生腹泻研究基金(IDF-2007-12)

摘　　要：目的了解乌鲁木齐地区婴幼儿腹泻轮状病毒(RV)感染流行情况。方法 2011年1～12月,采集门诊或住院≤5岁婴幼儿腹泻样本419例,用胶体金和ELISA法检测RV抗原,阳性样用逆转录-聚合酶链反应(RT-PCR)进行G、P基因型分型。结果 419例腹泻患儿,RV阳性率为24.3%(102/419),主要感染对象为24月以内婴幼儿(占92.2%)。对100份RV阳性样本进行G/P基因型分型,结果显示:G1和G9型为主要流行株,分别占26%,其次为G2型(19%)和G3型(11%);混合G型感染占7%,其中首次检到G4以混合感染形式存在3例;未能G分型达11%。P基因型以P[8]为主占55%,其次为P[4](21%)和P[6](16%);P型混合感染占6%;2例(2%)未能分型。最主要的G/P基因型组合依次为G1P[8](25%),G2P[4](18%),G9P[8](13%)、G9P[6](11%)和G3P[8](11%)。结论 2011年乌鲁木齐地区婴幼儿腹泻轮状病毒流行基因型多样,G9型和G2型明显上升,值得高度关注。持续监测优显重要。Objective To understand the epidemiological status of rotavirus diarrhea among children in Urumqi in 2011. Methods 419 fecal samples collected from children aged 5 years and under with diarrhea were test- ed for rotavirus antigen using Colloidal Gold method and ELISA in 2011. Rotavirus from positive samples were further genotyped by RT-PCR for G and P type. Results The overall positive rate of RV antigen was 24.3% and 92.2% of cases were infants of under 24 months old. G-typing showed that G1 （26%） and G9 （26%） were predominant, followed by G2 （19%） and G3 （11% ？）. 7% of cases were co-infected with dif- ferent G type. G4 type was first detected in 3 patients with mixed G infection in Urumqi. 11 （11%） were un- determined for G types. P-typing showed that P[8], P[4] and PE6] were found to be predominant accounting for 55%, 21% and 16% of P types. P mixed infection was 6%.Two （2%） remained undetermined for P types. The most common G/P combination were GiP[-8] （25%）, G2P[-4] （18%0）, GgP[-8] （13%）, GgP [6] （11%） and G3P[8] （11%） . Conclusions There are a variety of rotavirus genotypes observed in young children with diarrhea in Urumqi in 2011 with an increased amount of G9 and G2 infection which is wor- thy of attention. So a non-stopping monitor is critical for the prevention.

关 键 词：轮状病毒 腹泻 儿童 基因型 

分 类 号：R725.1[医药卫生—儿科]