中药复方对艰难梭菌毒素基因tcd A/tcd B体外表达的影响  

The influences of Chinese herbal compounds on in vitro expression of toxic genes tcd A/tcd B of Clostridium difficile

作　　者：舒青龙[1] 彭淑红[1] 封勇[1] 张志丽 谢燕飞[1] 周寿然[1] 

机构地区：[1]江西中医学院基础医学院,江西南昌330004 [2]宜春市职业技术学院护理学院,江西宜春336000

出　　处：《中国微生态学杂志》2013年第4期373-375,380,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(81001680);江西省自然科学基金(20122BAB205073);江西省卫生厅中医药项目(2011A141);江西省卫生厅科技项目(20122030)

摘　　要：目的探讨中药复方治疗抗生素相关性腹泻(antibiotic associated diarrhea,AAD)的药理机制。方法应用荧光定量PCR技术检测2种中药复方(参苓白术散和理中汤)对2株艰难梭菌临床分离株(A+B+和A-B+)体外培养下毒素基因表达的影响。结果在A+B+艰难梭菌中,参苓白术散组对tcd A和tcd B基因的表达均有显著或是完全抑制,理中汤也有显著的抑制;而对于A-B+艰难梭菌,2种中药复方则完全抑制tcd B基因的表达。结论通过影响毒素基因的表达可能是中药复方治疗AAD的药理学机制之一。Objective To explore the pharmacological mechanism of Chinese Traditional Medicine in treatment of antibiotic associated diarrhea(AAD).Methods Real-time quantitative PCR was used to examine the influences of two Chinese herbal compounds on the expression of toxic genes(tcd A and tcd B) in two strains of Clostridium difficile,A+B+ and A-B+,respectively.Results The expression of tcd A gene of A+B+ strain was obviously inhibited by the two Chinese herbal compounds Shenling Baizhu San(powder of ginseng,poria and atractylodes etc.) and Lizhong Tang(decoction containing ginseng,dried ginger,licorice and atractylodes),while the expression of tcd B gene in A+B+ and A-B+ strains was undetectable by real time quantitative PCR.Conclusion The expression of toxic genes is a potential pharmacological pathway of Chinese Traditional Medicine for treatment of AAD.

关 键 词：抗生素相关性腹泻 艰难梭菌 定量PCR 中药复方 药理机制 

分 类 号：R378.8[医药卫生—病原生物学]