微生态制剂对缺血性结肠炎大鼠肠道微生物和血脂的影响  

Effects of micro-ecological agents on intestinal microorganisms and serum lipid in rat models of ischemic colitis

作　　者：俞金莲[1] 马良[1] 

机构地区：[1]绍兴第二医院消化内科,浙江绍兴312000

出　　处：《中国微生态学杂志》2013年第4期396-398,共3页Chinese Journal of Microecology

摘　　要：目的探讨微生态制剂对缺血性结肠炎大鼠肠道微生物和血脂的影响。方法将30只SD大鼠制成缺血性结肠炎模型,并随机分为正常对照组、模型对照组和治疗组。正常对照组、模型对照组仅给予正常饲料喂食,治疗组在正常饲料喂食的基础上予复方嗜酸乳杆菌片灌胃(1×107CFU/d),4周后抽血化验大鼠总胆固醇及甘油三酯,并处死大鼠,取肠道内容物做肠道乳酸菌、大肠埃希菌及真菌等微生物培养,数据采用统计学方法分析。结果与正常对照组相比,模型对照组乳酸菌减少,差异有统计学意义(P<0.05),大肠埃希菌和真菌数量增加,差异有统计学意义(P<0.05);治疗组乳酸菌增多,差异有统计学意义(P<0.01),大肠埃希菌和真菌数量减少,差异有统计学意义(P<0.05)。同时,治疗组大鼠总胆固醇及甘油三酯降低,差异有统计学意义(P<0.05)。结论缺血性结肠炎者出现肠道微生物紊乱,微生态制剂能增加肠道乳酸菌等益生菌,减少肠道条件致病菌和致病菌,维持肠道微生态平衡,同时对患者的血脂有一定的调节作用。Objective To investigate the effect of micro-ecological agents on intestinal microorganisms and serum lipid in rat models of ischemic colitis.Methods 30 Sprague-Dawley(SD) rat models of ischemic colitis were randomly divided into three groups: the normal control group,the model control group and the treatment group.The normal control group and the model control group were treated with normal feeds only,while the treatment group were given normal feeds and compound eosinophil-lactobacillus tablets(1×107 CFU/d).Four weeks later,their cholesterol(CHOL) and triacylglycerol(TG) were determined,and intestinal contents were cultured to detect lactic acid bacteria,Escherichia coli and fungi.The experimental data were analyzed by statistical methods.Results Compared with the normal control group,the number of lactic acid bacteria was lower in the model control group(P<0.05),and the numbers of E.coli and fungi were higher(P<0.05);the number of lactic acid bacteria was significantly higher in the treatment group(P<0.01),and the numbers of E.coli and fungi were lower(P<0.05).Meanwhile,the levels of cholesterol(CHOL) and triacylglycerol(TG) were lower in the treatment group(P<0.05).Conclusion Micro-ecological agents can increase the amount of gut probiotics,decrease the amount of enteric pathogenic bacteria and conditional pathogens,and mainain the equilibrium of microecosystem in intestines.And meanwhile,they have a certain regulatory effect on serum lipid.

关 键 词：微生态制剂 缺血性结肠炎 SD大鼠 肠道微生物 血脂 

分 类 号：R378[医药卫生—病原生物学]