根田鼠粪便皮质酮的检测效能  

The utility of detecting corticosterone levels in feces of root vole (Microtus oeconomus)

作　　者：何慧[1,2] 曹伊凡[1] 陈黎林 堵守杨[1,2] 聂绪恒[1,2] 边疆晖[1] 

机构地区：[1]中国科学院西北高原生物研究所,中国科学院高原生物适应与进化重点实验室,西宁810001 [2]中国科学院大学,北京100049 [3]青海省地方病预防控制研究所,西宁811602

出　　处：《兽类学报》2013年第2期164-171,共8页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目(31170394)

摘　　要：为验证根田鼠粪便皮质酮的可检测效能,本研究检测根田鼠粪便皮质酮含量的昼夜变化,并检测急性应激后和慢性应激期间根田鼠粪便皮质酮含量变化,及其慢性应激个体的HPA轴负反馈功能。结果表明,根田鼠粪便皮质酮水平具有明显的似昼夜节律,粪便中皮质酮含量的最高点出现在08:00和24:00,最低点在12:00和16:00;在终止急性应激12h后,根田鼠粪便皮质酮含量显著增加,且有性别间差异;慢性应激根田鼠粪便皮质酮含量始终保持在高水平;再次急性应激,慢性应激根田鼠个体的粪便皮质酮含量较对照个体升高的时间延后。上述结果说明,根田鼠的粪便皮质酮含量能够反映机体所处的生理状态及应激水平,因此,该方法可用于野外根田鼠种群的相关研究并具有可靠性。To determine the utility of detecting corticosterone levels in feces of root vole （Microtus oeconomus）, fecal corti- costerone concentration during 24-hour rhythm, alteration induced by acute and chronic restraints and the negative feedback function of HPA axis were measured. The results showed that the fecal corticosterone concentration in root voles displayed dynamic diurnal changes. This index peaked at 08：00 and 24：00, and fallen to the bottoms at 12：00 and 16：00. Two hours of restraint caused a 12h-delayed significant increase in fecal eorticosterone, with sex-differences. The fecal cortieos- terone concentration of chronic stressed voles maintained at relatively high levels. Furthermore, the chronically stressed in- dividuals＇ stress reaction time is relatively long, indicating the negative feedback function of HPA axis was deteriorated. Based on the above, fecal cortieosterone measurements provide a reliable, accurate and useful method to assessing individu- al stress level. It can be assured use in studies related to root vole population.

关 键 词：根田鼠 粪便 皮质酮 日节律 束缚应激 

分 类 号：Q494[生物学—生理学]