大绒鼠NPY、AgRP、POMC和CART基因部分序列扩增与分析  

Amplification and sequence analysis of partial cDNA sequence of NPY, AgRP, POMC and CART genes in Eothenomys miletus

作　　者：黄春梅[1] 朱万龙[1] 杨盛昌[1] 余婷婷[1] 王政昆[1] 

机构地区：[1]云南师范大学生命科学学院,昆明650500

出　　处：《兽类学报》2013年第2期186-192,共7页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目(31071925;31260097);云南省教育厅科研基金资助项目(ZD2009007);云南省应用基础研究计划项目(2011FZ082)

摘　　要：下丘脑在维持能量代谢活动中发挥着关键的作用,下丘脑核团之间通过神经肽形成的调控网络是能量平衡的基础（焦广发等,2010）。下丘脑神经元会分泌一些神经肽。Neuropeptide Y （NPY）, agouti related peptide （AgRP）, pro-opiomelanocortin （POMC） and cocaine and am- phetamine regulated transcript peptide （CART） are four neuropeptides that play essential roles in the regulation of food in- take and energy homeostasis in mammals. Partial eDNA sequences of NPY, AgRP, POMC and CART genes were obtained using the RT-PCR method. The obtained partial sequences of NPY, AgRP, POMC, and CART were 162 bp, 187 bp, 240 bp, and 55 bp in length, respectively, which contain open reading frames （ORF） of 114 bp, 179 bp, 214 bp, 54 bp separately. The protein coding of these sequences include 37 amino acids in NPY; 59 amino acids in AgRP; 71 a- mino acids in POMC ; 17 amino acids in CART. A search of the BLAST database showed that the deduced sequence of E. miletus NPY, AgRP, POMC and CART were very similar to the NPY, AgRP, POMC and CART of Rattus norvegicus, Mus musculus, and Acomys russatus. In order to make a further determination of the evolutional position of E. miletus, the NPY, AgRP, POMC and CART genes were used to reconstruct phylogenetic trees by using ML method. The result showed that E. miletus has the closest relationship relative to R. norvegicus and M. museulus. The finding of this comparative study will contribute to further revealing the energy metabolism mechanisms of E. miletus from an evolutionary perspective.

关 键 词：大绒鼠 神经肽Y 刺鼠相关蛋白 阿片促黑色素原 可卡因-安他非明转录调节肽 下丘脑 

分 类 号：Q78[生物学—分子生物学]