和田羊毛囊KAP1.1基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of KAP1.1 gene of the hair follicles in Hetian sheep

作　　者：刘鑫[1] 李树伟[1] 姜仁军[1] 

机构地区：[1]塔里木大学生命科学学院塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300

出　　处：《黑龙江畜牧兽医》2013年第5期41-43,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30960272)

摘　　要：为了分析和田羊毛囊角蛋白关联蛋白(KAP)1.1基因,试验以南疆地方品种羊(和田羊)为样本,从和田羊毛囊中提取总RNA,设计1对引物,通过PCR反应扩增出长度为535 bp的基因片段,连接到pMD18-T载体上。结果表明:克隆得到和田羊毛囊KAP1.1基因成熟蛋白编码序列,序列长度与GenBank上发表的序列长度相同,经生物信息学软件分析,有2处碱基发生突变,同源性为99%,说明试验成功克隆了KAP1.1基因。To analyze the keratin- associated protein （KAP） 1.1 gene of the hair follicles in Hetian sheep, which was the local breed sheep in south region of Xinjiang, the Hetian sheep as a sample were selected to extract the total RNA from the hair follicles. A pair of primers was de- signed, and a 535 bp gene fragment was amplified by PCR, and then was connected into the pMD18 -T vector. The results showed that the se- quence of KAPI. 1 gene encoding mature protein in the hair follicles in Hetian Sheep was obtained, the length of its sequence was same as that of the sequence published on GenBank. Bioinformatics software analysis showed that there were two base mutations, and the homology was 99%. The results indicate that KAP1. 1 gene is cloned successfully.

关 键 词：和田羊毛囊 角蛋白关联蛋白(KAP)1 1基因 克隆 生物信息学分析 

分 类 号：Q785[生物学—分子生物学]