广东省麻风菌株基因分型研究  

Genotyping of Mycobacterium leprae in Guangdong province

作　　者：黎明[1] 王晓华[1] 苏婷[1] 邢燕[2] 翁小满[2] 

机构地区：[1]广东省皮肤性病防治中心,广州510095 [2]首都医科大学附属北京友谊医院北京热带医学研究所

出　　处：《中华流行病学杂志》2013年第5期424-427,共4页Chinese Journal of Epidemiology

基　　金：国家自然科学基金（3067111）;美国NIH/NIAID（R01-A1-63457）

摘　　要：目的了解广东省麻风菌株基因型及其传播与国内外的关系，探讨外来麻风病患者对广东省流行可能产生的影响。方法对广东省籍与外省籍麻风患者取皮肤活检，进行麻风菌株的可变数目串联重复序列（VNTR）与单核苷酸多态性（SNP）分型。结果广东籍患者主要以SNP1型菌株为主，少数为SNP3型菌株。输入性病例菌株均为SNP3型菌株。SNP1型菌株的VNTR基因型，尤其是18-8、12—5、ML-1、（TA）10与（GGT）位点的等位基因与国内其他地区SNP3型麻风菌株不同。但是SNP3型的广东籍患者其菌株的VNTR基因型与SNP1型菌株的VNTR型很接近，18-8、12—5等位基因型一致，差异仅在ML-1，（TA）10和（GCT）5位点。结论广东籍患者麻风菌株以SNP1为主，与国内多地区的菌株不同。推测其传播路径与早期“海上丝绸之路”有关。少数为SPN3型的广东籍患者是否为输入病例的二代传播，值得深入研究及监测追踪。Objective To understand the genotypes of Mycobacterium leprae collected from Guangdong province, China and to analyze the routes of leprosy transmission both inside and outside of Guangdong. The impact of emigrant leprosy patients to the endemic nature of the disease in Guangdong was also studied. Methods Typing on strains with variable number of tandem repeats （VNTR） and single nucleotide polymorphism （SNP） were performed on the local cases and emigrant cases based on skin biopsy. Results Most isolates from local patients belong to SNP type 1 and SNP type 3 isolates were found just in a small part of local isolates. However, all the emigrants were carrying SNP type 3. Within the SNP type 1 strain from Guangdong, alleles at the 18-8,12-5 ,ML-1, （TA） 10 and （GGT） 5 differed from SNP 3 strains collected from other areas in China. However, all the SNP type 1 and SNP type 3 local isolates identified from Guangdong were having close VNTR profiles and the main differences appeared in the alleles at ML-1, （TA） 10 and （GGT）5. Conclusion The transmission of strain with SNP type 1 seemed to be associated to the ＂Silk Road on the Sea＂, calling for monitoring and confirming the transmission of patients with SNP type 3 in Guangdong were from the secondary transmission, by the emigrant patients. Further study on the historic spread and phylogenetic relationships between SNP type 1 and novel SNP type 3 in Guangdong is needed.

关 键 词：麻风菌 基因分型 传播 

分 类 号：R755[医药卫生—皮肤病学与性病学]