非O157产志贺毒素大肠埃希菌分离株脉冲场凝胶电泳分析  

Pulsed-field gel electrophoresis typing on non-O157 Shiga toxin-producing Escherichia coli isolates

作　　者：金东[1] 赵爱兰[1] 白向宁[1] 孟琼[1] 于波[1] 袁雪娇[1] 熊衍文[1] 侯雪新[1] 李振军[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《中华流行病学杂志》2013年第5期503-506,共4页Chinese Journal of Epidemiology

摘　　要：目的对国内不同宿主来源的非O157产志贺毒素大肠埃希菌（STEC）分离株进行脉冲场凝胶电泳（PFGE）分析，以初步建立中国非O157STEC菌株的基础数据库，了解其分子流行病学特征。方法参照PulseNet非O157STEC的PFGE实验方法对76株非O157STEC分离株进行分析，并使用BioNumerics软件进行聚类。结果在PulseNet推荐的电泳参数和内切酶XbaI情况下，菌株酶切片段分布均匀，条带易于识别。76株非O157STEC分离株产生62种PFGE带型，初步聚类为A-M13个群，不同来源菌株的PFGE带型分布广泛，但均具有某些优势的PFGE聚类群。结论国内不同来源的非O157STEC呈高度多态性，PulseNet推荐的PFGE电泳参数和内切酶XbaI适用于中国非O157STEC菌株的分析。Objective To establish a database and to understand the molecular epidemiological features of non-O157 Shiga toxin-producing Escherichia coli （STEC） isolates from different animal reservoirs and patients. Methods Pulsed-field gel electrophoresis （PFGE） was performed according to the PulseNet protocol with minor modifications. A dendrogram was constructed using the BioNumerics. Results Under the PulseNet protocol, 62 PFGE patterns were obtained from 76 non-O157 STEC isolates and then divided into A to M groups. Isolates from different sources were widely distributed in different groups, but were predominant seen in certain groups. Conclusion The non-O157 STEC isolates in China were highly polymorphic. PulseNet protocol seemed to be suitable for the typing of Chinese non-O 157 STEC isolates.

关 键 词：非O157产志贺毒素大肠埃希菌 脉冲场凝胶电泳 分子分型 

分 类 号：R446.5[医药卫生—诊断学]