清心开窍方含药脑脊液对连二亚硫酸钠所致PC12细胞损伤的保护机制研究  

Study on protective effect of cerebrospinal fluid containing Qingxin Kaiqiao Fang on sodium dithionite-induced PC12 cell injury

作　　者：胡海燕[1] 崔志慧[1] 陈翔[1] 王文花[1] 张晓艳[1] 

机构地区：[1]温州医学院第二临床学院,浙江温州325003

出　　处：《中国中药杂志》2013年第9期1314-1317,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973780);浙江省自然科学基金项目(Y2080273);温州市科技局项目(Y20080106)

摘　　要：目的:从细胞水平研究清心开窍方含药脑脊液对连二亚硫酸钠(sodium dithionite,Na2S2O4)损伤的PC12细胞的保护作用,为该方剂的临床应用提供依据。方法:SD大鼠灌胃给予清心开窍方水煎液(7.9 g.kg-1),每12 h ig 1次,共7次,制备清心开窍方含药脑脊液。采用PC12细胞和终浓度8 mmol.L-1的Na2S2O4共培养,造成神经细胞损伤模型。以尼莫地平(1×107mol.L-1)为阳性对照组,MTT法检测清心开窍方含药脑脊液对PC12细胞活性的影响,RT-PCR方法检测Bax,Bcl-2,Caspase-3 mRNA表达水平。结果:清心开窍方含药脑脊液组PC12细胞活性明显高于模型组,Bax,Caspase-3 mRNA表达水平降低,Bcl-2 mRNA表达增高,与模型组比较差异显著(P<0.05,P<0.01)。结论:清心开窍方对Na2S2O4损伤的PC12细胞有明显的保护作用。Objective： To study the protective effect of cerebrospinal fluid containing Qingxin Kaiqiao Fang on sodium dithion- ite （Na2S2O4 ）-induced PCl2 cell injury, in order to provide basis for clinical application of the prescription. Method： SD rats were orally administered with water decoction of Qingxin Kaiqiao Fang （7.9 g · kg^-1 ） once every 12 h, for a total of 7 times, in order to prepare cerebrospinal fluid containing Qingxin Kaiqiao Fang. The neurocyte injury model was established by adding Na2S2 O4 with the final concentration of 8 mmol · L^-1 into PCl2 cells. With nimodipine （1 ×10^7mol·L^-1） as the positive control group, MTT method test was adopted to detect the impact of cerebrospinal fluid containing Qingxin Kaiqiao Fang on the activity of PCl2 ceils. The expres- sion of Bax, Bcl-2 and Caspase-3 mRNA was detected by RT-PCR. Result： The cerebrospinal fluid containing Qingxin Kaiqiao Fang groups showed a significantly higher activity in PCl2 cells than the model group, with decrease in expressions of Bax mRNA and Caspase-3 mRNA and increase in expression of Bcl-2 mRNA. There were significant differences compared with the model group （P 〈 0.05 ,P 〈 0. 01 ）. Conclusion： Qingxin Kaiqiao Fang shows a notable protective effect on Na2S2O4induced neurocyte injury.

关 键 词：清心开窍方 PC12细胞 连二亚硫酸钠 保护作用 

分 类 号：R285.5[医药卫生—中药学]