雌激素对大鼠宫颈扩张痛的影响  

Effect of estrogen on the pain of uterine cervical dilatation in rats

作　　者：李文会[1] 颜涛[1] 安光惠[1] 李士通[1] 汪正平[2] 

机构地区：[1]上海交通大学附属第一人民医院麻醉科,上海200080 [2]同济大学附属上海市第十人民医院麻醉科

出　　处：《上海医学》2013年第2期143-145,共3页Shanghai Medical Journal

摘　　要：目的探讨雌激素对大鼠宫颈扩张(UCD)痛的快速影响及机制。方法 40只雌鼠去卵巢术后14～20d,建立UCD模型。经大鼠尾静脉分别给予17-β雌二醇100ng(雌二醇组)、G蛋白耦联雌激素受体(GPR30)激动剂G-1300ng(G-1组)、GPR30抑制剂G-151000ng+17-β雌二醇100ng(G-15组)和含体积分数为0.1的二甲亚砜的聚丁二酸丁二醇酯液(溶剂对照组),4组药物的总容量均为1mL。比较给药前和给药后30min,在同样递增的宫颈拉力下腹直肌肌电反应的变化。结果实验过程中,有8只雌鼠对UCD无相关的肌电反应,将其排除后,4组各有8只雌鼠入组。4组间去卵巢术前及UCD模型建立前体重的差异均无统计学意义(P值均>0.05)。随着给予的宫颈拉力的递增,大鼠腹直肌肌电反应亦逐渐增大。雌二醇组和G-1组在20、40、60和80g宫颈拉力下给药前后的肌电反应变化百分率均显著高于溶剂对照组(P值均<0.05),而G-15组与溶剂对照组的差异均无统计学意义(P值均>0.05)。结论对于去卵巢术后的雌鼠,静脉给予雌激素能快速(30min)增加腹直肌对UCD的反应,给予GPR30激动剂对痛觉有增强作用,但在给予GPR30抑制剂后雌激素的这种增强作用被抑制,雌激素对UCD痛的短期影响可能是通过GPR30受体通路实现的。Objective To explore the fast effect and mechanism of estrogen on the pain of uterine cervical dilatation （UCD） in rats. Methods UCD models were established in 40 female rats 14 to 20 days after ovariectomization. The rats were given 100 ng 17-βestrogen （E2 group）, 300 ng G protein-coupled the estrogen receptor （GPR30） agonist G-1 （G-lgroup）, 1 000 ng GPR30 antagonist G-15 plus 100 ng estrogen （G-15 group）, or PBS solution including 0.1 dimethyl sulfoxide （control group） by caudal vein. The total volume of drugs in the four groups was 1 mL. Electromyogram changes of rectus abdominis were compared 30 mins before and after intravenous injection. Results Eight rats were excluded because there were no correlation between the UCD and myoelectricity reaction. Finally there were 8 rats in each group. Finally there were no significant diffenrecs in the rat weights among groups before ovariectomization and UCD modeling （all P〈0.05）. With the increasing of the UCD force, electromyogram （EMG） response of rectus abdominis increased gradually. EMG responses of rectus abdominis on the 20, 40, 60, and 80 g UCD force in the E2 group and G-1 group were significantly increased as compared with control group （all P〈0.05）. There was no significant difference in EMG response under the same forces between G-15 group and control group （all P〉0.05）. Conclusion Intravenous injection of estrogen can increase rectus abdominis EMG response to UCD quickly in ovariectomized rats. GPR30 agonist can enhance the effect, and GPR30 antagonist can suppress the effect. The short term effect of estrogen on UCD pain may be mediated by GPR30 receptor pathway.

关 键 词：雌激素 GPR30 内脏痛 宫颈扩张 

分 类 号：R96[医药卫生—药理学]