珍珠梅提取物TTF1抑制肝癌细胞增殖作用的研究  

Inhibition of HCC cells proliferation by TTF1 from extract of herbal medicine

作　　者：李光星[1] 林冬岩[2] 张学武[1] 

机构地区：[1]延边大学 [2]中国人民解放军空军93011部队

出　　处：《时珍国医国药》2013年第4期774-775,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30860374)

摘　　要：目的探讨珍珠梅提取物TTF1抑制肝癌细胞增殖作用的机制。方法利用MTT细胞毒性实验检测TTF1对人肝癌细胞HepG2和正常人永生化肝细胞Chang-liver增殖的影响,并采用EdU实验进行验证;通过Western Blotting实验在蛋白水平检测TTF1作用下,人肝癌细胞HepG2中常见的增殖指标-野生型p53以及Bcl-2变化情况。结果 MTT与EdU实验证实TTF1可以明显抑制人肝癌细胞HepG2的增殖,但对正常肝细胞Chang-liver毒性较小,野生型p53可随TTF1作用剂量增加而表达增高,而Bcl-2则随之降低。结论 TTF1可能通过上调野生型p53蛋白表达,抑制Bcl-2蛋白的表达而抑制肝癌细胞增殖,对正常细胞毒性较小,可能开发为新型毒副作用较小的肝癌药物。Objective To study the mechanisms of TTF1 inhibiting the proliferation of hepatocellular carcinoma(HCC) cells. Methods The inhibitory rate of TTF1 on HCC cells was detected by MTT on the normal human liver cell lines,Chang-liver,and the HCC cell line,HepG2.The effect of TTF1 on HCC cells proliferation was detected by EdU assay.And the protein expression levels of wild type p53 and Bcl-2 were examined by western blot. Results The MTT and EdU data showed that TTF1 inhibited HCC cell line HepG2 proliferation.Western blot analysis showed that TTF1 treatment led to decreased protein expression of bcl-2 and an increase in wild type p53. Conclusion These results together suggested that TTF1 may induce proliferation of HepG-2 cells through a complex pathway.

关 键 词：TTF1 肝癌细胞 增殖 

分 类 号：R284.2[医药卫生—中药学]