小半夏加茯苓汤中6-姜酚的含量测定  

Determination of 6-Gingerol in Xiaobanxiajiafuling Tang

作　　者：刘育辰[1] 刘刚[1] 田洪星[1] 何平康[1] 何前松[1] 冯泳[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《时珍国医国药》2013年第4期784-786,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(30960472);贵州省科技计划(黔科合中药字【2011】LK7030号)

摘　　要：目的研究以HPLC法测定小半夏加茯苓汤中6-姜酚含量的方法。方法采用Diamonsil C18柱(4.6 mm×250mm,5μm),以乙腈-0.1%磷酸水为流动相进行梯度洗脱,体积流量为1.0 ml.min-1,波长280 nm,柱温30℃。结果 6-姜酚在0.009 43～0.113 g.L-1与峰面积呈现良好的线性关系,r=0.999 8;平均加样回收率(n=6)为103.0%,RSD为0.89%。结论该方法操作简便,结果可靠,重复性好,可作为研究小半夏加茯苓汤中6-姜酚含量的方法。Objective To establish an HPLC method for determination of 6-gingerol in Xiaobanxiajiafuling Tang. Methods A Diamonsil C18 column(4.6 mm×250 mm,5 μm) was used with acetonitrile-0.1% phosphoric acid solution mixture in gradient mode with a flow rate of 1.0 mL·min-1,wavelength 280 nm,and the column temperature was at 30℃. Results The linear range of 6-gingerol was within 0.1886～2.2632 μg,r=0.9998,the average recovery was 103.0%,RSD was 0.89%. Conclusion This method has good reproducibility.It can be used for quality control in production of Xiaobanxiajiafuling Tang.

关 键 词：高效液相色谱法 小半夏加茯苓汤 6-姜酚 含量测定 

分 类 号：R284.2[医药卫生—中药学]