大黄酸及其金属配合物与牛血清白蛋白的相互作用  

Interaction of Bovine Serum Albumin with Rhein and its Metal Complexes

作　　者：周能[1] 覃苑[2] 周振[1] 沈丽[1] 

机构地区：[1]玉林师范学院化学与材料学院,广西玉林537000 [2]广西大学化学化工学院,广西南宁530004

出　　处：《时珍国医国药》2013年第4期788-790,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81060360);广西科学基金(No.2010GXNSFA013065);2010年广西高等学校优秀人才资助计划项目

摘　　要：目的研究大黄酸(RH)及其配合物与牛血清白蛋白(BSA)之间的相互作用。方法采用多种光谱技术测定,用Stern-Volmer方程等对数据进行处理。结果大黄酸及其配合物均能显著猝灭BSA的内源荧光并以静态猝灭为主。在293K和300K时,RH-Fe与BSA结合常数分别为3.55×105L/mol和2.46×105L/mol,RH-Co与BSA结合常数分别为4.83×105 L/mol和1.87×105 L/mol,RH-Mn与BSA结合常数分别为4.12×105 L/mol和1.83×105L/mol。根据热力学参数判断大黄酸及其配合物间力主要是范德华力和氢键;依据Frster的偶极-偶极非辐射能量转移理论,计算出大黄酸及其钴、铁、锰配合物在蛋白质中的结合位置与色氨酸残基间的距离分别为为2.22,2.57,3.14和2.59 nm。结果还表明在同一温度下,金属离子如钴离子、锰离子、铁离子的存在会影响大黄酸与BSA的结合。结论大黄酸形成配合物后与BSA的结合显著增强,会对大黄酸及金属离子的储运产生影响。Objective To study the interaction between bovine serum albumin(BSA) and rhein or its complexes. Methods Spectroscopic methods were utilized to determine the binding data.Stern-Volmer equation was used to process the data. Results Rhein and its complexes could significantly quench the endogenous fluorescence of BSA mainly by a static mode.The binding constants Kb of RH-Fe,RH-Co and RH-Mn with BSA are 3.55×105 L/mol,2.46×105 L/mol,4.83×105 L/mol,1.87×105 L/mol,3.55×105 L/mol and 2.46×105L/mol at 293K and 300K,respectively.From the thermodynamics parameter judge,we can learn that the major forces between rhein or its complexes and the BSA are vander walls force and hydrogen bond.Based on Frster non-radiation energy transfer mechanism,the binding site were found to be an area of 2.22,2.57.3.14 and 2.59 nm away from tryptophan residue in BSA for RH,RH-Co,RH-Fe and RH-Mn,respectively.The results also show that the binding constant of RH with BSA can be affected by coexistence of Co2+,Mn2+,Fe2+ in the same temperatun. Conclusion The binding ability of RH with BSA can significantly enhance after forming metal complexes and then affect the storage and transportation of RH and metal ions.

关 键 词：大黄酸 配合物 牛血清白蛋白 结合作用 光谱方法 

分 类 号：R284.2[医药卫生—中药学]