菩人丹对T2DM胰腺微循环损伤大鼠Ang-1、Ang-2/Tie-2的影响  

Regulation Effects of the Chinese Pu-Ren-Dan Recipe on Ang-1,Ang-2 and Tie-2 in Type 2 Diabetic Mellitus Rats with Pancreatic Microcirculation Disturbance

作　　者：白永飞[1] 杜子亮[1] 鲁碧楠[1] 陈书[1] 德力格玛[1] 庞宗然[1] 

机构地区：[1]中国少数民族传统医学国家民委-教育部重点实验室,北京100081

出　　处：《时珍国医国药》2013年第4期791-793,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30873249);北京市自然科学基金(No.7122091);中央民族大学自主科研项目(No.1112KYXJ09)

摘　　要：目的观察菩人丹(PuRenDan,PRD)对2型糖尿病(Type 2 Diabetes Mellitus,T2DM)胰腺微循环损伤大鼠胰腺组织中血管生成素-1、2(Angiopoietin-1,-2,Ang-1,-2)及可溶性酪氨酸激酶受体-2(Tyrosine Kinase Receptors-2,TIE-2)的影响,探讨PRD改善胰腺微循环,修复胰岛微血管损伤的分子机制。方法采用脂肪乳结合小剂量链脲佐菌素(streptozotocin,STZ)尾静脉快速推注的方法建立T2DM微循环损伤大鼠模型。成模大鼠随机分为T2DM模型组、菩人丹组(1.77 g.kg-1)和降糖通脉片组(0.42 g.kg-1),另随机选取10只正常大鼠作为正常对照组。各组动物每日灌胃给药1次,正常对照组和T2DM模型组给予等剂量蒸馏水,连续给药4周后,提取各组大鼠胰腺组织总蛋白,采用Westernblot法测定各组大鼠胰腺组织中Ang-1、Ang-2的蛋白质表达,及TIE-2蛋白磷酸化水平。结果与正常对照组比较,T2DM胰腺微循环损伤大鼠胰腺组织Ang-1蛋白质表达水平和TIE2磷酸化水平明显降低(P<0.01),而Ang-2蛋白质表达水平显著增高(P<0.01);菩人丹治疗后,Ang-1蛋白质表达水平和TIE2磷酸化水平均显著上调(P<0.01),而Ang-2蛋白质表达被抑制(P<0.01),其作用与阳性对照药降糖通脉片相当。结论菩人丹通过调控Ang-1、Ang-2/TIE-2蛋白表达及磷酸化,促进糖尿病状态下胰腺组织微血管生成,进而改善胰腺微循环,发挥其对胰岛β细胞损伤的修复作用。Objective To explore the molecular mechanism of PRD on improving pancreatic microcirculation and repairing pancreatic microvascular by observing the regulation effects of PRD on angiopoietin-1(Ang-1),angiopoietin-2(Ang-2) and tyrosine kinase receptors-2(Tie-2) of pancreas in T2DM rats with pancreatic microcirculation disturbance. Methods Experimental animal models of type 2 diabetes mellitus rats with pancreatic microcirculation disturbance were established by feeding with fat emulsion through stomach and injecting streptozotocin(STZ) through tail vein.Then model rats which were successfully replicated were randomized into model group,PRD group(1.77g·kg-1) and JiangTangTongMaiPian group(0.42g·kg-1) and selected 10 normal rats as control group.And rats of PRD group and JiangTangTongMaiPian group were given drugs by intragastric administration,while model group and control group were given stilled water.Four weeks later,total protein of each rat were collected.Using western blot assay to analyze the protein expression changes of Ang-1 and Ang-2 and phosphorylation levels of Tie-2 in each group. Results Protein expressions of Ang-1 and phosphorylation levels of Tie-2 were significantly decreased in T2DM group compared with the control group(P<0.01),while Ang-2 expression was significantly increased(P<0.01).After the treatment of PRD,the expressions of Ang-1 and Tie-2 were up-regulated(P< 0.01),and Ang-1 was down-regulated significantly(P< 0.01),which was equivalent to the positive drugs. Conclusion PRD could regulate protein expressions of Ang-1 and Ang-2 and phosphorylation levels of Tie-2,which made PRD could repair injured β cell in T2DM through improving pancreatic microcirculation which owing to accelerating generation of pancreatic microvascular.

关 键 词：菩人丹 胰腺微循环障碍 血管生成素-1 血管生成素-2 可溶性酪氨酸激酶受体-2 

分 类 号：R284[医药卫生—中药学]