松针叶绿素-胡萝卜素软膏体外清除自由基及体内抗氧化作用的研究  

Study on free radial scavenging capacity and antioxidation of chlorophyll-carotene paste from pine needles in vitro and in vivo

作　　者：郑光耀[1] 何玲[2] 薄采颖[1] 谢衡 刘冬芹[2] 谢学容 

机构地区：[1]中国林业科学研究院林产化学工业研究所.生物质化学利用国家工程实验室 [2]国家林业局林产化学工程重点开放性实验室,江苏南京210042 [3]中国药科大学药理学教研室,江苏南京210009

出　　处：《时珍国医国药》2013年第4期815-817,共3页Lishizhen Medicine and Materia Medica Research

基　　金：科技部农业科技成果转化资金项目(No.2008GB24320412);2010年教育部"新世纪优秀人才"支持计划资助项目(No.NCET-10-0817);国家"十二五"重大新药创制专项(No.2012ZX09504001-001)

摘　　要：目的研究松针叶绿素-胡萝卜素软膏体外清除自由基和体内抗氧化作用。方法通过测定松针叶绿素-胡萝卜素软膏对羟自由基(·OH)、超氧阴离子自由基(O2-·)和DPPH自由基(DPPH·)的清除率,观察松针叶绿素-胡萝卜素软膏体外清除自由基能力;通过测定大鼠血清、肝匀浆和心匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量变化,观察松针叶绿素-胡萝卜素软膏体内抗氧化作用。结果松针叶绿素-胡萝卜素软膏对·OH、O2-·、DPPH.均具有明显的清除能力,其IC50分别为0.53,0.23,0.56 mg/ml;能显著提高大鼠血清、肝脏和心脏中SOD、GSH-Px活性和降低MDA含量。结论松针叶绿素-胡萝卜素软膏在体内外具有良好的抗氧化活性,为松针叶绿素-胡萝卜素软膏在食品和药物中的应用提供实验依据。Objective To study on free radial scavenging capacity and antioxidation of chlorophyll-carotene paste from pine needles. Methods The free radical scavenging capacity of chlorophyll-carotene paste was evaluated by measuring the scavenging rate of hydroxyl radical,superoxide radical and DPPH radical in vitro.The antioxidant activity of chlorophyll-carotene paste was analyzed by measuring SOD,GSH-Px and MDA concentrations in serum,liver and heart of rat in vivo. Results The chlorophyll-carotene paste showed high scavenging capacity against the hydroxyl,superoxide and DPPH radicals with IC50 values of 0.53,0.23 and 0.56 mg/ml,respectively.The chlorophyll-carotene paste could significantly increase the activities of SOD and GSH-Px while decrease the content of MDA in serum,liver and heart of rat. Conclusion Chlorophyll-carotene paste from pine needles might be a valuable antioxidant of natural source that could be applicable in the food and medicine industry.

关 键 词：松针 提取物 自由基 抗氧化 

分 类 号：R284.2[医药卫生—中药学]