针刺对哮喘大鼠肺组织p38蛋白激酶及c-fos蛋白表达的影响  

The Effects of Acupuncture on P38 MAKP and C-fos Expression in the Lung Tissues of Asthmatic Rats

作　　者：杨晓溪[1] 崔建美[1] 喇孝瑾[1] 孙娜[1] 刘慧娟[1] 王洪彬[1] 林清[1] 包巨太[1] 

机构地区：[1]河北联合大学中医学院,河北唐山063000

出　　处：《时珍国医国药》2013年第4期1004-1006,共3页Lishizhen Medicine and Materia Medica Research

基　　金：河北省自然科学基金(No.C2010001797)

摘　　要：目的探讨针刺对哮喘大鼠肺组织中p38MAPK、c-fos蛋白表达的影响。方法将SPF级雄性SD大鼠随机分为空白组、哮喘模型组、哮喘模型针刺组3组,每组10只。制作卵蛋白致敏大鼠哮喘模型。采用HE染色法、免疫组化法,观察3组大鼠肺组织病理变化及p38MAPK、c-fos蛋白的表达程度。结果针刺组大鼠肺组织HE染色病理改变较哮喘模型组有所减轻,但较空白组重。免疫组化结果显示,针刺组大鼠肺组织p38MAPK、c-fos蛋白表达(AOD值)低于哮喘模型组(P<0.01)。结论针刺法具有良好的抗炎作用,能有效缓解大鼠哮喘发作。该法能明显抑制哮喘大鼠肺组织p38MAPK、c-fos的表达。其治疗作用机制:可能与MAPK接受细胞外针刺刺激发生反应,又参与细胞内的信息传递过程有关。Objective To examine this mechanism: we observed the effects of acupuncture on p38 MAKP and c-fos expression in the lung tissues of asthmatic rats. Methods Thirty adult male rats SPF grade were randomly divided into blank group,asthmatic model group and acupuncture model group by random number table: each group consisted of 10.Asthma rat model sensitized and challenged by ovalbumin was established.Light microscope was used to observe the effects of acupuncture on p38 MAKP and c-fos expression with HE staining and immunohistochemistry method. Results HE staining of rat lung indicated that the pathological changes of acupuncture group were very slight than asthmatic model group,but were worse than those of blank group.Immunohistochemistry showed that the protein expression of p38 and c-fos(AOD) in lung tissue in acupuncture group were lower than that in the asthmatic model group(P<0.01). Conclusion This needling has a significant anti-inflammatory effect,and can effectively relieve the clinical symptoms of asthmatic rats.This method can effectively decrease the lung tissues p38 MAPK and c-fos contents,and its mechanism perhaps is related to MAPK which plays an important role in the regulation of transferred signals between intracellular and extracellular by acupuncture stimulating.

关 键 词：哮喘 P38丝裂原活化蛋白激酶 C-FOS蛋白 针刺 免疫组化 

分 类 号：R2-03[医药卫生—中医学]