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作 者:杜旭[1] 王瑞辉[1] 张秋红[1] 王孟林[1] 陆建[1]
机构地区:[1]陕西中医学院,陕西咸阳712046
出 处:《时珍国医国药》2013年第4期1011-1014,共4页Lishizhen Medicine and Materia Medica Research
基 金:国家自然科学基金(No.81072869/H2718);陕西省中医管理局科研项目(No.09Jc61)
摘 要:目的探讨电针对大鼠坐骨神经损伤后神经导向因子Slit2及其mRNA表达的影响。方法健康SD大鼠72只,随机分为空白对照组、模型对照组和电针治疗组,每组24只。后两组大鼠右坐骨神经横断后即刻端对端缝合。电针治疗组取"环跳"、"足三里"进行电针治疗,每天1次,7 d为1个疗程,连续治疗3个疗程。每疗程结束后应用免疫组化法、逆转录-聚合酶链反应(RT-PCR)分别检测坐骨神经和相应脊髓段Slit2及其mRNA的表达变化。结果电针治疗组与模型对照组比较,大鼠损伤的坐骨神经和相应脊髓段中Slit2及其mRNA在第一疗程后达到高峰,之后逐渐降低,且都高于模型组,有非常显著性差异(P<0.01),而且两组始终都高于空白对照组,也有非常显著性差异(P<0.01)。结论电针治疗能明显增强损伤的坐骨神经和相应脊髓段中Slit1及其mRNA的表达,可能是其治疗周围神经损伤的机制之一。Objective To approach to the effects on nerve growth guidance factor Slit2 of rats after sciatic nerve injury. Methods 72 healthy SD rats were randomly divided into control group,model group and the electroacupuncture(EA) group(n = 24).In the latter two groups,the right sciatic nerve was transected and end-to-end suture immediately.The treatment of EA on 'Huantiao'(GB 30) and' Zusanli'(ST 36) was applied once daily,and 7 days a course of treatment,continuous treatment of three courses.The changes in expression of Slit2 and Slit2 mRNA in sciatic nerve and corresponding section of spinal cord were detected by RT-PCR and immunohistochemical methods after the end of each course of treatment. Results EA group compared with model group,Slit2 and its mRNA in the injured rat sciatic nerve and spinal cord segments reached a peak after the first course of treatment,then decreased gradually,and are higher than the model group,there was a remarkably significant difference(P<0.01),and the two groups is always higher than the control group,there are also remarkably significant differences(P<0.01). Conclusion EA treatment can remarkably strengthen expression of Slit2 and Slit2 mRNA in sciatic nerve and corresponding section of spinal cord after sciatic nerve injury.This may be the one of the mechanisms for the treatment of peripheral nerve injury.
关 键 词:电针 坐骨神经损伤 神经生长导向因子Slit2
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