荧光猝灭法测定中草药中痕量钴  

Fluorospectrophotometric Determination of Trace Amount of Cobalt in TCM

作　　者：陶慧林[1] 黎舒怀[1] 徐铭泽[1] 周素莲[1] 韦柳丹[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,桂林541004

出　　处：《理化检验（化学分册）》2013年第4期413-416,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：广西自然基金(2011GXNS-FA018061);国家自然基金(21167006)

摘　　要：在pH 7.4的磷酸盐缓冲溶液和十六烷基三甲基溴化铵(CTMAB)介质中,钙黄绿素与罗丹明B之间发生能量转移而导致后者的荧光增强(λem581nm)。当在此体系中加入痕量Co2+后,于发射波长581nm处产生荧光猝灭现象。据此提出了荧光猝灭法测定中草药中痕量钴含量。Co2+的质量浓度在4.0×10-4～1.35×10-2g.L-1范围内与反应液荧光强度的降低程度呈线性关系,方法的检出限(3S/N)为1.8×10-7g.L-1。方法用于中草药样品分析,回收率在97.6%～102%之间,测定值的相对标准偏差(n=6)在0.98%～1.5%之间。In a PBS of pH 7.4 and in the presence of CTMAB, reaction of energy transfer between rhodamine B and caleein occurred as observed by the enhancement of fluorescence intensity at the ;tern of 581 nrn, When microamount of Co2＋ was added to this reaction system, significant decrease in fluorescence intensity at this wavelength was observed. Based on these facts, a method for the determination of trace amount of Co2＋ by fluorospectrophotometry was proposed. Linear relationship between the magnitude of decrease in fluorescence intensity and mass concentration of Co2＋ was obtained in the range of 4.0×10^-4～1.35×10^-2g·L^-1, with detection limit （3S/N） of 1.8×10^-7g·L-1. The proposed method was used to the analysis of TCM, giving values of recovery and RSD＇s （n=6） in ranges of 97.6%-102% and 0. 98%--1.50% respectively.

关 键 词：荧光猝灭法 钙黄绿素 罗丹明B 中草药 钴 

分 类 号：O657.31[理学—分析化学]