高效液相色谱法测定透明质酸中乙二胺四乙酸  

HPLC Determination of EDTA in Hyaluronic Acid

作　　者：耿小锋 高明 徐艳林 李明慧 

机构地区：[1]华勤橡胶工业集团研发实验室,兖州272100

出　　处：《理化检验（化学分册）》2013年第4期442-444,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：提出了高效液相色谱法测定透明质酸中乙二胺四乙酸(EDTA)含量的方法。用ZOR-BAX Eclipse XDB-C18(4.6mm×150mm,5μm)色谱柱分离,以乙腈-pH 6.5的100g.L-1四丁基氢氧化铵-水(20+20+60)混合溶液为流动相,用紫外检测器在波长260nm处测定。EDTA的质量浓度在0.037 2～1.86mg.L-1范围内呈线性,检出限(3S/N)为4.745μg.L-1。方法用于分析透明质酸样品,回收率在87.2%～93.6%之间,测定值的相对标准偏差(n=5)小于5%。HPLC was applied to the determination of EDTA in hyaluronic acid. ZORBAX Eclipse XDB-G8 column （4. 6 mm×150 mm, 5 μm） was used as stationary phase and a mixture of acetonitrile, 100 g·L^-1 tetrabutyl ammonium hydroxide of pH 6. 5 and water （20 to 20 to 60 by vol. ） was used as mobile phase. UV- detection at the wavelength of 260 nm was adopted. Linear relationship between values of peak area and mass concentration of EDTA was kept in the range of 0. 037 2-1. 86 mg·L^-1, with detection limit （3S/N） of 4. 745 μg·L^-1. The proposed method was used in the analysis of sample hyaluronic acid, values of recovery found were in the range of 87.2%--93.6% with RSD＇s （n=5） less than 5%.

关 键 词：高效液相色谱法 透明质酸 乙二胺四乙酸 

分 类 号：O652.63[理学—分析化学]