钩藤生物碱调节肠系膜动脉细胞增殖凋亡相关蛋白的研究  

Regulation effect of uncaria alkaloids on proliferation apoptosis related protein of mesenteric arterial cells

作　　者：焦华琛[1] 李运伦[1] 孙敬昌[2] 

机构地区：[1]山东中医药大学附属医院,山东济南250011 [2]山东中医药大学基础医学院细胞生物学实验室,山东济南250355

出　　处：《山东中医药大学学报》2013年第3期244-247,共4页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(编号:81072794);山东省中药现代化科技专项资助课题(编号:2008GG2NS02011)

摘　　要：目的:研究钩藤碱、异钩藤碱和钩藤总生物碱对自发性高血压大鼠(SHR)肠系膜动脉平滑肌细胞凋亡和增殖的影响。方法:将SHR分组,分别给予钩藤碱、异钩藤碱和钩藤总生物碱,并以Wistar大鼠作为正常对照。采用HE染色法观测肠系膜动脉的病理形态,采用Masson染色法观察肠系膜动脉胶原含量,采用免疫组化法检测肠系膜动脉Bcl-2、Bax、c-myc、c-fos、MMP-9和TIMP-2的蛋白表达。结果:钩藤碱、异钩藤碱和钩藤总生物碱能减轻SHR肠系膜动脉的病理损害,降低动脉壁的胶原沉积,下调肠系膜动脉平滑肌Bcl-2、c-fos和TIMP-2蛋白表达,钩藤碱上调肠系膜动脉MMP-9蛋白表达。结论:钩藤碱和异钩藤碱可能通过下调原癌基因Bcl-2蛋白表达,促进SHR肠系膜动脉细胞凋亡,通过下调c-fos蛋白表达途径抑制SHR肠系膜动脉细胞异常增殖,并通过调节MMP-9和TIMP-2蛋白表达抑制SHR肠系膜动脉的胶原沉积,从而改善肠系膜动脉壁重塑。Objective ：To study the apoptosis and proliferation effect of rhynchophylline ,isorhynehophylline and uncaria alkaloids on spontaneously hypertensive rat（SHR） mesenterie artery smooth muscle cells. Methods：SHR were divided into different groups, and given rhynehophylline,isorhynchophylline and unearia alkaloids. Wistar rats were used as normal control. Mesenterie artery pathology was observed by HE staining,mesenterie artery collagen content by Masson staining. Bcl-2,Bax,c-myc,c-fos,MMP-9 and TIMP-2 protein expression of mesenterie artery were detected by immunohistoehemieal. Resuhs：Rhynchophylline,isorhyn- chophylline and unearia alkaloids could alleviate the pathological damage of the SHR mesenterie arteries,reduce arterial wall collagen deposition,and lower the protein expression of Bel-2,e-fos and TIMP-2 of mesenterie artery smooth muscle. Rhynchophylline increased mesenteric artery MMP-9 protein expression. Conelusion：Rhynchophylline and isorhynehophylline may promote apoptosis of SHR mesenteric arteries by lowering the proto-oneogene Bcl-2 protein expression,inhibite mesenterie artery cells abnormal proliferation by down-regulating e-fos protein expression pathway,and inhibite collagen deposition through regulating MMP-9 and TIMP-2 protein expression. Thereby the mesenteric arterial walls were improved.

关 键 词：钩藤提取物 高血压 肠系膜动脉 凋亡 增殖 大鼠 实验研究 

分 类 号：R285.5[医药卫生—中药学]