不同输液方案对兔肺组织水通道蛋白5表达的影响  

Effect of different infusion modes on the expression of aquaporin-5 in the lung of rabbits

作　　者：檀俊涛[1] 徐红萌[1] 贾慧群[1] 王勇[1] 赵爱华[1] 李超[1] 王合梅[1] 邱东洁[1] 

机构地区：[1]河北医科大学附属第四医院,石家庄05001

出　　处：《军事医学》2013年第3期223-226,230,共5页Military Medical Sciences

基　　金：河北省卫生厅医学科学研究重点课题计划(07115)

摘　　要：目的观察不同输液方案对兔肺组织水通道蛋白5(AQP5)表达的影响。方法清洁级成年雄性SD家兔54只,随机分为3组(n=18):限制性输液组(A组,输液速度5 ml/h),常规输液组(B组,输液速度10 ml/h),急性高容量血液稀释组(C组,输液速度20 ml/h)。各组动物分别于输液前(T0)、输液后30 min(T1)、输液后60 min(T2)、输液后120 min(T3)记录心率(HR)、平均动脉压(MAP)、中心静脉压(CVP)等血流动力学指标。将A组在T1、T2、T3时刻再分为3组(n=6):A0.5组(T1)、A1.0组(T2)、A2.0组(T3),B、C组再分组时刻同A组。然后,分别于T1、T2、T3时刻处死动物,取相同部位右肺组织制作HE切片,光镜观察肺组织结构变化;同时取肺组织标本于透射电镜下观察肺组织超微结构变化。免疫组化染色检测AQP5的表达,图像分析测定AQP5染色的平均灰度和阳性单位。结果光镜下各组实验动物肺组织无病理学改变;透射电镜观察各组实验动物肺组织超微结构无变化。免疫组化和图像分析结果显示,肺泡Ⅰ型上皮细胞有大量AQP5阳性染色;不同输液方式对AQP5平均灰度和阳性单位的影响差异无统计学意义(P﹥0.05)。结论不同输液方式对肺组织AQP5的表达无显著影响。Objective To observe the effect of different infusion modes on the expression of aquaporin-5 in the lung of rabbits. Methods Fifty-four healthy adult male Clean SD rabbits were randomly divided into 3 groups （n = 18） ： restricted fluid administration group （group A, transfusion speed 5 ml/h） ;conventional fluid administration group （group B, l0 ml/ h） and AHH （ group C, 15 ml/h）. HR, MAP, CVP were recorded before infusion （To）, and 30, 60, 120 min after in-fusion （T1-3 ）. Group A was divided into 3 subgroups （n =6） at T1, T2, T3 ： group A0.5, group A1.0 and group A2.0. Group B and C were divided in the same way. The rabbits were sacrificed, and the same part of pulmonary tissues was taken out at T1-3. The changes of lung tissues were observed under a light microscope and transmission electron microscope. The expression of AQPs was determined by immunohistochemical staining test ,while the value of the average gray scale and posi- tive unit was determined by image analysis. Results There was no difference in lung tissues under the light microscope or transmission electron microscope between the groups. A large amount of AQP5 positive staining was found in Type I alveolar epithelial cells by immunohistochemistry and image analysis technology. No difference was found in the value of A and posi- tive unit of AQP5 in different infusion modes （P 〉 0.05 ）. Conclusion Different infusion modes have no effect on the ex- pression of AQP5 in the lung of rabbits.

关 键 词：水通道蛋白质5 肺泡Ⅰ型上皮细胞 限制性补液 急性高容量血液稀释 

分 类 号：R619[医药卫生—外科学]