电化学发光免疫传感器检测相思子毒素研究  

Research on abrin detection by electrochemiluminescence immunosensor

作　　者：刘冰[1] 童朝阳[1] 郝兰群[1] 穆晞惠[1] 刘威[1] 黄启斌[1] 

机构地区：[1]防化研究院国民核生化灾害防护国家重点实验室,北京102205

出　　处：《传感器与微系统》2013年第5期28-30,34,共4页Transducer and Microsystem Technologies

基　　金：总装预先研究资助项目(404070205)

摘　　要：以磁性微球固定相思子毒素多抗制备捕获探针,以三联吡啶钌标记相思子毒素单抗作为发光探针,两者与相思子毒素发生特异性免疫反应形成夹心复合物,成功建立了相思子毒素的电化学发光免疫传感检测方法。利用此方法检测相思子毒素,浓度在0.1～1000μg/L范围内与电化学发光强度呈良好的对数线性关系,拟合方程为lg Y=0.763 lg X+0.562(R=0.9903,N=7,P<0.0001),检出限为0.1μg/L。Polyclonal antibody（PcAb）of abrin is immobilized on magnetic microbeads as capture probe.An efficient approach for abrin detection by electrochemiluminescence（ECL）immunosensor is developed based on specific immunological reaction between immobilized PcAb,abrin and Ru（bpy）2＋3-labeled monoclonal antibody（McAb）,which forms sandwich immunocomplex.Then the target abrin is determined by ECL.A good logarithmic linear relationship between ECL intensity and the concentration of abrin at a range of 0.1 ~1 000 μg/L is obtained,fitting equation is lg Y=0.763 lg X＋0.562（R=0.990 3,N=7,P0.000 1）and the detection limit is 0.1 μg/L.

关 键 词：电化学发光 免疫传感器 检测 相思子毒素 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]