羟基红花黄色素A对抗原诱导RBL-2H3细胞活化脱颗粒的影响  

Effects of Hydroxysafflor Yellow A on Antigen-stimulated Degranulation of Basophilic Cell Leukemia Cell Line in Rats

作　　者：黄丰[1] 李满萍[1] 吴赛春[1] 涂苑青[1] 曾静[1] 童晓云[2] 

机构地区：[1]暨南大学药学院中药药理教研室,广州510632 [2]云南中医学院第一附属医院,昆明650021

出　　处：《中国药房》2013年第19期1738-1741,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81073129)

摘　　要：目的:研究羟基红花黄色素A(HYSA)对抗原诱导大鼠嗜碱性细胞白血病细胞株(Rat basophilic leukemiacell line,RBL-2H3)细胞脱颗粒的影响。方法:采用MTT法检测HYSA对RBL-2H3细胞增殖活性的影响;每孔加入抗DNP-IgE单抗(0.25μg/ml)与二硝基苯基人血清白蛋白(30ng/ml)以复制抗原诱导细胞脱颗粒模型,研究HYSA对抗原诱导RBL-2H3细胞活化脱颗粒的抑制作用;采用Westernblot法检测HYSA对抗原诱导RBL-2H3细胞活化脱颗粒丝裂原活化蛋白激酶(MAPK)蛋白表达的影响。结果:3～300μmol/L的HYSA对RBL-2H3细胞的增殖无明显影响;300μmol/LHYSA能显著降低RBL-2H3细胞脱颗粒程度以及抑制MAPK蛋白的表达。结论:HYSA对抗原诱导RBL-2H3细胞活化脱颗粒的炎症反应具有一定的抑制作用,且其作用可能与抑制MAPK蛋白表达有关。OBJECTIVE： To study the effects of Hydroxysaffior yellow A （HYSA） on antigen-stimulated degranulation in RBL-2H3 cells. METHODS： MTT assay was performed to establish the effect of HYSA on the RBL-2H3 cells viability; the RBL-2H3 cells were stimulated with 0.25 μg/ml anti-DNP IgE and 30 ng/ml DNP-HSA to establish degranulation model, the inhibitory effect of HYSA on antigen-stimulated degranulation in RBL-2H3 cells was researched; MAPK protein expression after antigen-induced degranulation was examined by the Western blot analysis in RBL-2H3 cells. RESULTS： HYSA（3-300 μmol/L）had no significant effect on RBL-2H3 cells proliferation. HYSA （300 μmol/L）significantly decreased the degranulation and MAPK phosphorylated protein level in RBL-2H3 cells. CONCLUSIONS：HYSA inhibited the inflammation on antigen-stimulated degranulation in RBL-2H3 ceils through inhibiting of the MAPK protein phosphorylation.

关 键 词：羟基红花黄色素A 大鼠嗜碱性细胞白血病细胞株 脱颗粒 

分 类 号：R285.5[医药卫生—中药学]