金丝桃苷对高糖和氧化损伤所致内皮细胞凋亡的影响  

Effects of Hyperoside on the Apoptosis of Endothelial Cells Due to High Glucose and Oxidative Damage

作　　者：吴畏[1] 于彩平[1] 李卓恒[1] 刘岭[2] 孟德胜[1] 

机构地区：[1]第三军医大学第三附属医院药剂科,重庆400042 [2]第三军医大学统计学教研室,重庆400038

出　　处：《中国药房》2013年第19期1744-1747,共4页China Pharmacy

基　　金：重庆市卫生局中医药科研计划立项项目(No.2011-2-116)

摘　　要：目的:研究金丝桃苷(HP)对高糖和氧化损伤所致内皮细胞凋亡的影响。方法:体外培养内皮细胞,分为正常细胞、正常细胞+HP、高糖损伤、高糖+HP、H2O2损伤与H2O2+HP组,采用MTT法测定内皮细胞的活力,并采用RT-qPCR法、Westernblot法分别测定B细胞淋巴瘤/白血病-2基因(Bcl-2)蛋白及其mRNA表达水平,Bcl-2相关X蛋白(Bax)及其mRNA表达水平。结果:与各自损伤组比较,高糖+HP、H2O2+HP组内皮细胞损伤程度显著减轻(P<0.01),高糖+HP、H2O2+HP组内皮细胞Bcl-2mRNA和蛋白表达显著增强(P<0.01),BaxmRNA和蛋白表达显著减弱(P<0.01)。结论:HP通过抑制Bax的表达,上调Bcl-2的表达而拮抗高糖、氧化损伤导致的内皮细胞凋亡,对糖尿病性视网膜病变血管内皮细胞具有一定的保护作用。OBJECTIVE： To study the effects of hyperoside（HP） on the apoptosis of endothelial cells due to high glucose and oxidative damage. METHODS： Endothelial cells were cultured in vitro and divided into normal cells, normal＋HP, high glucose in- jury, high glucose＋HP, H202 injury and H202 injury ＋HP groups. MTT assay was used to determine the activity of endothelial cells, and RT-qPCR method and Western blot assay were adopted to detect mRNA and protein expression of Bcl-2 and Bcl-2 related X protein （Bax）. RESULTS： Compared with injury group, the injury of endothelial cells relieved significantly in high glucose＋ HP group and H20~ injury ＋HP group（P〈0.01 ）. mRNA and protein expression of Bcl-2 increased significantly in high glucose＋HP group and H202 injury ＋HP group （P〈0.01）, while the mRNA and protein expression of Bax decreased significantly （P〈0.01）. CONCLUSION： By inhibiting the expression of Bax and up-regulating the expression of Bcl-2 , HP can antagonize apoptosis of en- dothelial cells due to high glucose or oxidative damage. HP has a orotective effect on DR vascular endothelial cells.

关 键 词：金丝桃苷 高糖 氧化 凋亡 B细胞淋巴瘤 白血病 2基因 B-细胞淋巴瘤白血病-2基因相关X蛋白 

分 类 号：R285.5[医药卫生—中药学]