何首乌不同提取物清除羟自由基的活性研究  

Study on the Hydroxyl Radical Scavenging Activity of Different Extracts of Polygonum multiflorum

作　　者：李奇[1] 王伽伯[2] 肖小河[2] 赵奎君[1] 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]解放军第302医院全军中医药研究所,北京100039

出　　处：《中国药房》2013年第19期1760-1762,共3页China Pharmacy

摘　　要：目的:研究何首乌不同提取物清除羟自由基的活性。方法:采用分析羟化产物法对何首乌不同提取物清除羟自由基能力与半数清除浓度(LC50)进行比较,结合何首乌不同提取物化学成分分析,归属清除羟自由基可能的有效化学成分。结果:何首乌不同提取物清除羟自由基能力从高到低与LC50从低到高均依次为醇提水沉物、醇提物、醇提水溶物/总提物、水提醇溶物、水提物、水提醇沉物,即何首乌醇提水沉物清除羟自由基活性最高。结论:大黄酸可能是清除羟自由基的有效化学成分。应重点关注醇提水沉操作,以使何首乌在清除羟自由基方面发挥更大功效。OBJECTIVE： To study the hydroxyl radical scavenging activity of different extracts of Polygonum multiflorum. METHODS： The capabilities of different extracts of P. multiflorum in scavenging hydroxyl radical and LC50 were studied and com- pared by analysis of hydroxylation products. Combined with chemical components analysis of different extracts of P. multiflorum, the potential active chemical components of hydroxyl radical scavenging were attributed. RESULTS：The capability of hydroxyl radi- cal scavenging in descending order and LC50 in ascending order, alcohol extraction and water-precipitation, alcohol extraction, alcohol extraction and water-soluble or total extraction, water extraction and alcohol-soluble, water extraction, water extraction and alcohol- precipitation, i.e. the alcohol extraction and water-precipitation had the highest scavenging activity. CONCLUSIONS： Rhein may be the active chemical components of scavenging hydroxyl radical. We should focus on the method of alcohol extract-water precipitation so that P. multiflorum may play a greater effect on the field of scavenging hydroxyl radical.

关 键 词：何首乌 醇提物 醇提水沉 清除羟自由基活性 有效化学成分 

分 类 号：R932[医药卫生—生药学] R97[医药卫生—药学]