天麻素对Ox-LDL诱导牛主动脉内皮细胞黏附HL-60细胞的抑制作用  

Inhibitory effect of gastrodine on the Ox-LDL-stimulated adhesion of HL-60 cells to bovine artery endothelial cells

作　　者：于滨[1] 桂冠[2] 杨欣 张娜[1] 王真[1] 李电东[1] 邓洪斌[1] 

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所,北京100050 [2]南京医科大学第一附属医院,南京210029 [3]陕西省食品药品检验所,西安710061

出　　处：《中国新药杂志》2013年第9期1014-1018,共5页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2012ZX09301002-001-015);国家自然科学基金(81170326;81072645)

摘　　要：目的:研究天麻素对Ox-LDL诱导牛主动脉内皮细胞(BAECs)黏附HL-60细胞的抑制作用及其机制。方法:通过细胞黏附实验观察天麻素对BAECs与HL-60细胞的黏附作用;流式细胞术检测ICAM-1和VCAM-1的表达;细胞色素C还原法测定细胞含量;报告基因法检测核转录因子κB(NF-κB)转录活性的变化。结果:天麻素可使Ox-LDL诱导的BAECs黏附HL-60细胞显著降低,BAECs细胞表面ICAM-1和VCAM-1表达下降,细胞含量以及NF-κB转录活性也显著下降。结论:天麻素能抑制Ox-LDL诱导的BAECs黏附HL-60细胞。Objective： To investigate the inhibitory effect of gastrodine on Ox-LDL-stimulated adhesion of HL-60 cells to bovine artery endothelial cells （BAECs） and the underlying mechanism. Methods： Cell adhesion assay was used to determine the effect of gastrodine on Ox-LDL-stimulated adhesion of HL-60 cells to BAECs. Flow cytometry was used to evaluate the expression of ICAM-1 and VCAM-1 on BAECs. Cytochrome C reduction assay was used to investigate the O2^- level in the cells, and lueiferase reporter gene assay was used to detect the NF-KB transcription activity. Results： As compared with the Ox-LDL-stimulated group, gastrodine significantly decreased the adhesion rate of HL-60 cells to BAECs, the expression levels of ICAM-1 and VCAM-1, and the level and the transcription activity of NF-κB. Conclusion： Gastrodine suppresses Ox-LDL-stimulated adhesion of HL-60 cells to BAECs.

关 键 词：低密度脂蛋白 天麻素 细胞黏附 核转录因子ΚB 

分 类 号：R972[医药卫生—药品]