音乐电针对慢性应激抑郁模型大鼠海马单胺类神经递质表达的调节作用  

Regulative effects of music electroacupuncture on expressions of hippocampus monoamine neurotransmitters in rats with chronic stress depression

作　　者：唐银杉[1] 余仁锋[1] 纪倩[1] 李国民[2] 姚海江[1] 莫雨平[1] 李志刚[1] 

机构地区：[1]北京中医药大学针灸推拿学院北京100029 [2]湖南省郴州市第一人民医院康复医学科

出　　处：《北京中医药大学学报》2013年第4期263-267,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81072860)

摘　　要：目的观察音乐电针对慢性应激抑郁模型大鼠海马单胺类神经递质五羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)表达的影响,探讨音乐电针治疗抑郁症的作用机制。方法 SD大鼠随机分为4组:空白组、模型组、氟西汀组、音乐电针组,除空白组外,其余均采用慢性应激结合孤养的方式造模。采用旷场实验、糖水消耗量检测和体重测量进行行为学评价;采用放射免疫法检测大鼠海马5-HT、NE、DA的表达。结果与空白组比较,模型组大鼠水平穿越格数、竖立次数、糖水消耗量、体重明显降低(P<0.01);与模型组比较,氟西汀组、音乐电针组水平穿越格数、竖立次数、糖水消耗量、体重均明显升高(P<0.05)。与空白组相比,模型组大鼠海马组织5-HT、DA、NE的含量明显降低(P<0.01);与模型组相比,氟西汀组大鼠海马组织的5-HT、DA、NE的含量升高(P<0.05);音乐电针组大鼠海马组织的5-HT、DA、NE的含量升高(P<0.01、P<0.05)。与氟西汀组比较,音乐电针组大鼠海马组织的5-HT的含量增加,有显著性差异(P<0.05)。结论音乐电针可以改善慢性应激抑郁大鼠的行为学症状,具有一定的抗抑郁作用;它能提高慢性应激模型大鼠中枢单胺类神经递质5-HT、DA、NE的水平,这可能是音乐电针发挥抗抑郁治疗作用的途径之一。Objective To observe the influences of music electroacupuncture on hippocampus monoamine neurotransmitters, 5-hydroxytryptamine （5-HT）, dopamine （DA） and norepinephrine （NE）, in rats with chronic stress depression. Methods SD rats were randomly divided into blank group, model group, flouxetine group and music electroacupuncture group. The rat model of chronic stress depression was established by applying chronic stress combining separation in all groups except blank group. The behavior changes of rats were reviewed through open-field test, sucrose consumption detection and body weight measure. The expressions of 5-HT, DA and NE were detected by using radioimmunoassay. Results Compared with blank group, number of horizontally crossed grids, standing times, sucrose consumption and body weight decreased significantly in model group （P 〈 0.01 ）. Compared with model group, number of horizontally crossed grids, standing times, sucrose consumption and body weight increased significantly in flouxetine group and music electroacupuncture group （P 〈 0.05）. Compared with blank group, the content of 5-HT, DA and NE decreased significantly in model group （P 〈 0.01 ）. Compared with model group, the content of 5-HT, DA and NE increased in flouxetine group （P 〈 0. 05 ）, and increased in music electroacupuncture group （P 〈 0.01, P 〈 0.05）. Compared with flouxetine group, the content of 5-HT increased in music electroacupuncture group （P 〈 0.05）. Conclusion Music electroacupuncture can relieve the ethologic symptoms in rats with chronic stress depression, so it has anti-depression effect. Music electroacupuncture can improve the levels of 5-HT, DA and NE, which may be one of its anti-depression mechanisms.

关 键 词：音乐电针 慢性应激 抑郁症 五羟色胺 多巴胺 去甲肾上腺素 大鼠 

分 类 号：R285.5[医药卫生—中药学]