基于SSR标记的草莓品种亲缘关系分析  

Genetic Relationships of Strawberry Cultivars by SSR Analysis

作　　者：韩柏明[1] 赵密珍[1] 王静[1] 于红梅[1] 

机构地区：[1]江苏省农业科学院园艺研究所,南京210014

出　　处：《植物遗传资源学报》2013年第3期428-433,共6页Journal of Plant Genetic Resources

基　　金：江苏省农业科学院博士后基金资助项目(6511006);江苏省自主创新项目(CX(11)1010)

摘　　要：用18对SSR引物对96份草莓栽培品种资源进行扩增。在18个SSR位点共获得184个条带,其中多态性条带为172个,多态性条带比率为93.5%,不同引物扩增的多态性条带数为4～19个,平均为9.56个。各位点多态性信息含量(PIC)值在0.5726～0.8885之间,平均0.8066。NTSYS软件进行相似性系数计算,UPGMA法进行聚类分析结果显示,96份来源不同的草莓品种被混杂地聚在一起,草莓品种的亲缘关系与其来源并不存在明显的相关性,中国地方品种的聚类相对集中,中国地方品种与中国选育品种之间亲缘关系相对较远。欧美品种和日本品种的遗传基础要宽于中国选育品种和地方品种。Eighteen SSR primer pairs were used to amplify SSR fragments for 96 strawberry cultivars. The 18 SSR primers generated a total of 184 bands among which 172 （93.5%）bands were found to be polymorphic. The number of polymorphic bands amplified per primer pair ranged from 4 to 19,with a average of 9.56. The value of locus polymorphism information content（ PIC） ranged from 0. 5726 to 0. 8885 ,with a average of 0. 8066. The genetic similarity revealed by NTSYS and the clustering results of UPGMA showed that these 96 strawberry cuhivars from different origins could not separately clustered well. There was no significant correlation between the genetic relationships and the origins of the cuhivars. The cluster of Chinese landraces was relatively concentrated ,while the genetic relationships between Chinese landraces and the developed cultivars in China were relatively farther. The genetic variations of the Euramerican cultivars and Japanese cultivars were more obvious than those of the Chinese landraces and the developed cuhivars in China.

关 键 词：草莓 亲缘关系 SSR 

分 类 号：S668.4[农业科学—果树学]