TcLr45凝集素类受体蛋白激酶基因克隆与序列分析  

Cloning and Sequence Analysis of Lectin-like Receptor Kinases Gene in TcLr45

作　　者：刘春燕[1,2] 魏学军[1] 张娜[1] 郭楠[1] 杨文香[1] 闫红飞[1] 刘大群[1] 

机构地区：[1]河北农业大学植物病理系/河北省农作物病虫害生物防治工程技术研究中心,保定071001 [2]河北北方学院动物科技学院,张家口075131

出　　处：《植物遗传资源学报》2013年第3期486-491,共6页Journal of Plant Genetic Resources

基　　金：国家重点基础研究发展计划(2013CB127700);国家公益性行业(农业)科研专项(200903035);教育部博士点基金(2010130212005);河北省留学人员科技活动项目择优资助项目(20120340);河北省教育厅自然科学青年基金(2011144)

摘　　要：在小麦抗叶锈近等基因系TcLr45中,扩增出一条凝集素类受体蛋白激酶基因片段。以该序列为靶序列,进行3'和5'RCAE-PCR,获得该基因cDNA全长片段为2347 bp,该序列具有完整的开放阅读框,共编码730个氨基酸,命名为LecRK-LR45。经软件分析,LecRK-LR45具有典型的凝集素类受体蛋白激酶结构,属于L类型。聚类分析结果表明,LecRK-LR45与山羊草和小麦抗叶锈近等基因系TcLr34中的凝集素类受体蛋白激酶基因具有较近的亲缘关系,与玉米、拟南芥的亲缘关系较远。该基因的克隆为研究小麦中凝集素类受体蛋白激酶的功能奠定了基础。A fragment of lectin-like receptor kinases gene was amplified in wheat leaf rust near-isogenic line TcLr45. The full length cDNA was 2347bp, which obtained using 3＇and 5＇RACE-PCR, baseing on the target sequence. This sequence had a full open reading frame （ORF） , encoding 730 amino acids, named as LeeRK-LR45. The LecRK-LR45 had a typical structure of lectin-like receptor kinases and belonged to L type. Cluster analysis result indicated that LecRK-LR45 had a closer genetic relationship with lectin-like receptor kinases gene of Aegilops tausehii and wheat（TcLr34） ,and farther with maize and arabidopsis. The cloning of this gene lied a foundation for researching the function of leetin-like receptor kinases in wheat.

关 键 词：小麦 凝集素类受体蛋白激酶(LecRKs) TcLr45 RACE 

分 类 号：S512.1[农业科学—作物学]